Endophytic fungal mutualists:: Seed-borne Stagonospora spp. enhance reed biomass production in axenic microcosms

被引：81

作者：

Ernst, M ^{[1
]}

Mendgen, KW ^{[1
]}

Wirsel, SGR ^{[1
]}

机构：

[1] Univ Konstanz, Fachbereich Biol, Lehrstuhl Phytopathol, D-78457 Constance, Germany

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2003年 / 16卷 / 07期

关键词：

ITS phylogeny; Phaeosphaeria;

D O I：

10.1094/MPMI.2003.16.7.580

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fungal endophytes mainly belong to the phylum Ascomycota and colonize plants without producing symptoms. We report on the isolation of seed-borne fungal endophytes from Phragmites australis (common reed) that were ascribed to the genus Stagonospora. Nested polymerase chain reaction (PCR) assays revealed that a Stagonospora sp. regularly colonized reed as shown for a period of three years. In spring, it was only detected in roots, whereas in autumn, it could frequently be found in all organs, including seeds. Microcosm experiments revealed that seeds harbored viable propagules of the fungus that colonized the developing germling, indicating vertical transmission. Endophytic growth was confirmed by immunofluorescence microscopy, reisolation of the fungus after surface sterilization, and PCR. Aseptic microcosms were established for studying fungal contributions towards host vitality. Several Stagonospora isolates enhanced reed biomass. Seed-borne endophytic Stagonospora spp. thus can provide improved vigor to common reed, which could be most important when seed-derived germlings establish new reed stands.

引用

页码：580 / 587

页数：8

共 26 条

[1] RELATIONSHIPS AMONG NON-ACREMONIUM SP FUNGAL ENDOPHYTES IN 5 GRASS SPECIES [J].

AN, ZQ ;

SIEGEL, MR ;

HOLLIN, W ;

TSAI, HF ;

SCHMIDT, D ;

SCHARDL, CL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (05) :1540-1548

[2] EFFECT OF THE TALL FESCUE ENDOPHYTE ON PLANT-RESPONSE TO ENVIRONMENTAL-STRESS [J].

ARACHEVALETA, M ;

BACON, CW ;

HOVELAND, CS ;

RADCLIFFE, DE .

AGRONOMY JOURNAL, 1989, 81 (01) :83-90

[3] HEREDITARY (SEED-BORNE) SYMBIOSIS IN CASUARINA-EQUISETIFOLIA FORST [J].

BOSE, SR .

NATURE, 1947, 159 (4041) :512-514

[4] THE SYMBIOTIC SEED-BORNE FUNGUS IN THE CISTACEAE .1. DISTRIBUTION AND FUNCTION OF THE FUNGUS IN THE SEEDING AND IN THE TISSUES OF THE MATURE PLANT [J].

BOURSNELL, JG .

ANNALS OF BOTANY, 1950, 14 (54) :217-+

[5] FUNGAL ENDOPHYTES IN STEMS AND LEAVES - FROM LATENT PATHOGEN TO MUTUALISTIC SYMBIONT [J].

CARROLL, G .

ECOLOGY, 1988, 69 (01) :2-9

[6]

Clay Keith, 1992, Natural Toxins, V1, P147, DOI 10.1002/nt.2620010302

[7] Taxonomy and identification of Septoria and Stagonospora species on small-grain cereals [J].

Cunfer, BM ;

Ueng, PP .

ANNUAL REVIEW OF PHYTOPATHOLOGY, 1999, 37 :267-284

[8] Interaction between the endophytic fungus Epichloe bromicola and the grass Bromus erectus:: effects of endophyte infection, fungal concentration and environment on grass growth and flowering [J].

Groppe, K ;

Steinger, T ;

Sanders, I ;

Schmid, B ;

Wiemken, A ;

Boller, T .

MOLECULAR ECOLOGY, 1999, 8 (11) :1827-1835

[9] An arbuscular mycorrhizal fungus accelerates decomposition and acquires nitrogen directly from organic material [J].

Hodge, A ;

Campbell, CD ;

Fitter, AH .

NATURE, 2001, 413 (6853) :297-299

[10] Linking plants to rocks: ectomycorrhizal fungi mobilize nutrients from minerals [J].

Landeweert, R ;

Hoffland, E ;

Finlay, RD ;

Kuyper, TW ;

van Breemen, N .

TRENDS IN ECOLOGY & EVOLUTION, 2001, 16 (05) :248-254

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