The utility of siRNA transcripts produced by RNA polymerase I in down regulating viral gene expression and replication of negative- and positive-strand RNA viruses

被引：66

作者：

McCown, M

Diamond, MS

Pekosz, A

机构：

[1] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA

[2] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA

[3] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA

来源：

VIROLOGY | 2003年 / 313卷 / 02期

关键词：

influenza A virus; West Nile virus; RNA interference; antivirals; gene expression;

D O I：

10.1016/S0042-6822(03)00341-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Short interfering double-stranded RNAs (siRNAs) expressed under the control of an RNA polymerase I promoter system were used to target gene expression of influenza A and West Nile virus. Decreased RNA and protein expression was induced in a sequence-specific manner-reducing sequence complementarity from 21 to 17 nucleotides abrogated the siRNA effect. Reduced M-2 expression resulted in a decrease in total and the expression of single viral genes, global viral gene expression, as a potential antiviral treatment, and as a genetic tool for viruses whose genomes are difficult to manipulate. (C) 2003 Elsevier Science (USA). All rights reserved.

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收藏

页码：514 / 524

页数：11

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