Visualization of dendrimer molecules by transmission electron microscopy (TEM): Staining methods and Cryo-TEM of vitrified solutions

被引：201

作者：

Jackson, CL ^{[1
]}

Chanzy, HD ^{[1
]}

Booy, FP ^{[1
]}

Drake, BJ ^{[1
]}

Tomalia, DA ^{[1
]}

Bauer, BJ ^{[1
]}

Amis, EJ ^{[1
]}

机构：

[1] NIST, Div Polymers, Gaithersburg, MD 20899 USA

来源：

MACROMOLECULES | 1998年 / 31卷 / 18期

关键词：

D O I：

10.1021/ma9806155

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Individual dendrimer molecules of poly(amidoamine) (PAMAM) from generation 10 (G10) to G5 were imaged by conventional transmission electron microscopy (TEM) after staining with aqueous sodium phosphotungstate. The dendrimers were resolved as separate, beam-stable entities, and their sizes and distribution of sizes were statistically analyzed and compared with data from small-angle X-ray scattering in solution. Aqueous G10 dendrimers were also characterized by examination in the frozen, hydrated state after quench-freezing in liquid ethane (cryo-TEM) and the data compared with the results of the staining experiments. To a first approximation, the dendrimers appeared circular in projection and the diameters conformed to a Gaussian distribution which broadened somewhat with increasing generation number. Cryo-TEM, in general, confirmed the staining experiments but suggested that, in the native state, there is more variability in the shapes of the dendrimers, with polyhedral shapes occurring quite frequently.

引用

页码：6259 / 6265

页数：7

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