Differentiation method-dependent expression of leptin in adipocyte cell lines

被引:22
作者
Slieker, LJ [1 ]
Sloop, KW [1 ]
Surface, PL [1 ]
机构
[1] Eli Lilly & Co, Lilly Res Labs, Endocrine Res Div, Indianapolis, IN 46285 USA
关键词
D O I
10.1006/bbrc.1998.9433
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Leptin, the product of the ob gene, is expressed exclusively in adipose tissue, However, adipocyte cell lines, such as 3T3-L1 adipocytes, have generally been reported to express extremely low levels of leptin mRNA. We compared 3T3-L1's to the closely related Line 3T3-F442A, and to another murine adipocyte line, TA1. TA1 cells, when differentiated by indomethacin/insulin treatment, express leptin at levels greater than those of 3T3-L1 adipocytes differentiated by the traditional methylisobutylxanthine/dexamethasone/insulin protocol. How ever, when 3T3-L1's are differentiated in the presence of indomethacin/insulin their expression levels of leptin increase dramatically. 3T3-F442A preadipocytes also express high levels of leptin when differentiated in the presence of T-3 and insulin, but when differentiated in the presence of indomethacin/insulin, expression levels drop precipitously. These changes in leptin mRNA and protein expression are not reflected by changes in CCAAT/enhancer binding protein-alpha (c/EBP alpha), peroxizomal proliferator activated receptor-gamma CPPAHy), lipoprotein lipase (LPL), fatty-acid binding protein aP2 or uncoupling protein-2 (UCP2) mRNA levels, and suggest a mechanism unique to the leptin gene. (C) 1998 Academic Press.
引用
收藏
页码:225 / 229
页数:5
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