An artificial aspartic proteinase system

被引：16

作者：

Jiang, L

Liu, ZL

Liang, Z

Gao, YH

机构：

[1] Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2005年 / 13卷 / 11期

关键词：

crown ether derivatives; carboxyl; aspartic proteinases; artificial enzymes; nucleophilic catalytic mechanism;

D O I：

10.1016/j.bmc.2005.03.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of aza crown ether derivatives with or without carboxyl groups in their side arms were synthesized and the former showed deacylation activities toward amino acid p-nitrophenyl ester hydrohalides. Substrate-selective phenomena were also observed. The relationship between the structures and deacylation activities of corresponding compounds suggested a nucleophilic catalytic mechanism. The results partially simulate some aspartic proteinases in the case of catalytic mechanism and are also useful for us to understand the detailed catalytic process of aspartic proteinases. (c) 2005 Elsevier Ltd. All rights reserved.

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页码：3673 / 3680

页数：8

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