Transcriptomic Analysis of Haematococcus lacustris during Astaxanthin Accumulation under High Irradiance and Nutrient Starvation

被引：35

作者：

Kim, Dong-Keon ^{[1
]}

Hong, Seong-Joo ^{[2
]}

Bae, Jae-Han ^{[2
]}

Yim, Narae ^{[2
]}

Jin, EonSeon ^{[3
]}

Lee, Choul-Gyun ^{[1
,2
]}

机构：

[1] Inha Univ, Dept Energy Engn, Inchon 402751, South Korea

[2] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea

[3] Hanyang Univ, Dept Life Sci, Seoul 133791, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2011年 / 16卷 / 04期

关键词：

Haematococcus lacustris; astaxanthin; cDNA microarray; transcriptomics; high irradiance; nutrient starvation; ISOPRENOID BIOSYNTHESIS; ESCHERICHIA-COLI; PLUVIALIS; EXPRESSION; ALGA; STRESS; PATHWAY; PROTEIN; CHLOROPHYCEAE; DIPHOSPHATE;

D O I：

10.1007/s12257-011-0081-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, a cDNA microarray was developed from over 60,000 mRNA readings to analyze the expression profiles of transcriptomes of Haematococcus lacustris under astaxanthin-inducing culture conditions, high irradiance and nitrate starvation. Among 20,033 genes on the cDNA microarray, 2,675 genes exhibited a twofold or greater difference in expression. Of these, 1,333 genes were up-regulated and 1,342 genes were down-regulated. A significant decrease in the expression of chlorophyll biosynthesis and light harvesting complex (LHC) related genes were observed under astaxanthin inducing conditions (forming red cyst cells). On the other hand, respiration-related genes, lipid metabolism-related genes and stress response-related genes were activated in the red cyst cells under stress conditions. These results enabled a better understanding of the cell responses during stress induction of H. lacustris such as photosynthesis, respiration, and some biopathways.

引用

页码：698 / 705

页数：8

共 24 条

[1]

BENOIT S, 2001, FEBS LETT, V500, P125

[2] Cell wall-specific ω-hydroxy fatty acids in some freshwater green microalgae [J].

Blokker, P ;

Schouten, S ;

Van den Ende, H ;

De Leeuw, JW ;

Damsté, JSS .

PHYTOCHEMISTRY, 1998, 49 (03) :691-695

[3] Phosphatidylglycerol depletion affects photosystem II activity in Synechococcus sp PCC 7942 cells [J].

Bogos, Balazs ;

Ughy, Bettina ;

Domonkos, Ildiko ;

Laczko-Dobos, Hajnalka ;

Komenda, Josef ;

Abasova, Leyla ;

Cser, Krisztian ;

Vass, Imre ;

Sallai, Anna ;

Wada, Hajime ;

Gombos, Zoltan .

PHOTOSYNTHESIS RESEARCH, 2010, 103 (01) :19-30

[4]

Boussiba S, 2000, PHYSIOL PLANTARUM, V108, P111, DOI 10.1034/j.1399-3054.2000.108002111x./

[5] Gene expression profile analysis in astaxanthin-induced Haematococcus pluvialis using a cDNA microarray [J].

Eom, H ;

Lee, CG ;

Jin, E .

PLANTA, 2006, 223 (06) :1231-1242

[6] Synthesis of (E)-4-hydroxydimethylallyl diphosphate.: An intermediate in the methyl erythritol phosphate branch of the isoprenoid pathway [J].

Fox, DT ;

Poulter, CD .

JOURNAL OF ORGANIC CHEMISTRY, 2002, 67 (14) :5009-5010

[7] Haematococcus astaxanthin:: applications for human health and nutrition [J].

Guerin, M ;

Huntley, ME ;

Olaizola, M .

TRENDS IN BIOTECHNOLOGY, 2003, 21 (05) :210-216

[8]

Kim JD, 2006, J MICROBIOL BIOTECHN, V16, P1222

[9] Gene expression profiling of Dunaliella sp acclimated to different salinities [J].

Kim, Minjung ;

Park, Seunghye ;

Polle, Juergen E. W. ;

Jin, EonSeon .

PHYCOLOGICAL RESEARCH, 2010, 58 (01) :17-28

[10] Astaxanthin biosynthesis enhanced by reactive oxygen species in the green alga Haematococcus pluvialis [J].

Kobayashi, M .

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2003, 8 (06) :322-330

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