rQuant.web: a tool for RNA-Seq-based transcript quantitation

被引:46
作者
Bohnert, Regina [1 ]
Raetsch, Gunnar [1 ]
机构
[1] Max Planck Gesell, Friedrich Miescher Lab, Tubingen, Germany
关键词
EXPRESSION;
D O I
10.1093/nar/gkq448
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
We provide a novel web service, called rQuant.web, allowing convenient access to tools for quantitative analysis of RNA sequencing data. The underlying quantitation technique rQuant is based on quadratic programming and estimates different biases induced by library preparation, sequencing and read mapping. It can tackle multiple transcripts per gene locus and is therefore particularly well suited to quantify alternative transcripts. rQuant. web is available as a tool in a Galaxy installation at http://galaxy.fml.mpg.de. Using rQuant. web is free of charge, it is open to all users, and there is no login requirement.
引用
收藏
页码:W348 / W351
页数:4
相关论文
共 22 条
[1]
ANDERSEN E. D., 2009, TR20093 MOSEK APS
[2]
Blankenberg Daniel, 2010, Curr Protoc Mol Biol, VChapter 19, DOI 10.1002/0471142727.mb1910s89
[3]
BOHNERT R, 2010, MODELLING B IN PRESS
[4]
Transcript quantification with RNA-Seq data [J].
Bohnert, Regina ;
Behr, Jonas ;
Raetsch, Gunnar .
BMC BIOINFORMATICS, 2009, 10 :P5
[5]
Optimal spliced alignments of short sequence reads [J].
De Bona, Fabio ;
Ossowski, Stephan ;
Schneeberger, Korbinian ;
Raetsch, Gunnar .
BIOINFORMATICS, 2008, 24 (16) :I174-I180
[6]
Substantial biases in ultra-short read data sets from high-throughput DNA sequencing [J].
Dohm, Juliane C. ;
Lottaz, Claudio ;
Borodina, Tatiana ;
Himmelbauer, Heinz .
NUCLEIC ACIDS RESEARCH, 2008, 36 (16)
[7]
Galaxy: A platform for interactive large-scale genome analysis [J].
Giardine, B ;
Riemer, C ;
Hardison, RC ;
Burhans, R ;
Elnitski, L ;
Shah, P ;
Zhang, Y ;
Blankenberg, D ;
Albert, I ;
Taylor, J ;
Miller, W ;
Kent, WJ ;
Nekrutenko, A .
GENOME RESEARCH, 2005, 15 (10) :1451-1455
[8]
Biases in Illumina transcriptome sequencing caused by random hexamer priming [J].
Hansen, Kasper D. ;
Brenner, Steven E. ;
Dudoit, Sandrine .
NUCLEIC ACIDS RESEARCH, 2010, 38 (12) :e131
[9]
Massively parallel sequencing of the polyadenylated transcriptome of C. elegans [J].
Hillier, LaDeana W. ;
Reinke, Valerie ;
Green, Philip ;
Hirst, Martin ;
Marra, Marco A. ;
Waterston, Robert H. .
GENOME RESEARCH, 2009, 19 (04) :657-666
[10]
Statistical inferences for isoform expression in RNA-Seq [J].
Jiang, Hui ;
Wong, Wing Hung .
BIOINFORMATICS, 2009, 25 (08) :1026-1032