Effects of lysophosphatidic acid on proliferation of stellate cells and hepatocytes in culture

被引：69

作者：

Ikeda, H

Yatomi, Y

Yanase, M

Satoh, H

Nishihara, A

Kawabata, M

Fujiwara, K

机构：

[1] Saitama Med Sch, Dept Internal Med 3, Moroyama, Saitama 35004, Japan

[2] Univ Tokyo, Dept Internal Med 1, Bunkyo Ku, Tokyo 113, Japan

[3] Yamanashi Med Univ, Dept Lab Med, Tamaho, Yamanashi 40938, Japan

[4] Japanese Fdn Canc Res, Inst Canc, Dept Biochem, Toshima Ku, Tokyo 170, Japan

[5] Japan Soc Promot Sci, Res Future Program, Toshima Ku, Tokyo 170, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 248卷 / 02期

基金：

日本学术振兴会;

关键词：

D O I：

10.1006/bbrc.1998.8983

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lysophosphatidic acid (LPA) is a growth factor-like mediator for fibroblasts or smooth muscle cells produced and released by activated platelets. Platelet activation occurs with hepatic necrosis and subsequent liver regeneration and fibrosis. In the fibrosis, hepatic stellate cells proliferate with phenotypic transformation to myofibroblasts, Thus, effects of LPA on proliferation of hepatocytes and stellate cells were investigated. In cultured rat stellate cells, LPA increased DNA synthesis with enhanced MAP kinase activity. Pertussis toxin (PTX) attenuated this mitogenic action. In contrast, LPA decreased DNA synthesis by cultured rat hepatocytes induced by hepatocyte growth factor (HGF) or epidermal growth factor (EGF) without affecting protein synthesis. Enhanced MAP kinase activity by HGF or EGF was not changed by LPA. This anti-mitogenic action was attenuated by PTX TGF beta level in the medium was less than the level effective for inhibiting the DNA synthesis in the presence of LPA Our results suggest that LPA might affect proliferation of hepatocytes and stellate cells in liver diseases complicating platelet activation. (C) 1998 Academic Press.

引用

页码：436 / 440

页数：5

共 51 条

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