Effect of triiodothyronine administration on estrogen receptor contents in peripuberal Sertoli cells

The effects of thyroid hormone on androgen metabolism in peripuberal Sertoli cells through the inhibition of estradiol production have been reported previously. It was our intention to investigate further the possible role of thyroid hormone on the interaction between testicular steroids and Sertoli cells by analyzing the effects of triiodothyronine (T-3) On. estrogen receptor content in 2-, 3- and 4-week-old euthyroid rats. Triiodothyronine treatment (3 mu g/100 body wt per day) given during the last week prior to sacrifice resulted in reduced testicular growth in 2-week-old animals. Sertoli cells from all groups were cultured initially under basal conditions for the first 24 h and subsequently in the presence of testosterone and/or T-3 for the additional 24 h. The in vitro addition of T-3 induced a decrease of estrogen receptors (ERs) in 2- and 3-week-old animals that appeared more pronounced especially in the presence of T-3 and testosterone. When T-3 was tested in vivo we noticed that the decrease of ER content was even greater in all three groups under the in vitro influence of both T-3 and testosterone. In 3-week-old animals a simultaneous assay of ERs in both nuclear and cytoplasmic compartments was performed. The ER concentrations in the nucleus were closely related to those of the cytoplasm. The in vivo administration of T-3 was responsible for a greater decrease of ERs in the nucleus than in the cytosol. On the basis of these results, and in agreement with our previous data, we speculate that the effect of T-3 in the maturational events of Sertoli cells could involve both estradiol production and ER content.