Antigenic characterization of canine parvovirus strains isolated in Italy

被引：49

作者：

Sagazio, P ^{[1
]}

Tempesta, M ^{[1
]}

Buonavoglia, D ^{[1
]}

Cirone, F ^{[1
]}

Buonavoglia, C ^{[1
]}

机构：

[1] Fac Vet Med, Dipartimento Sanita Patol Farmacotossicol & Benes, I-70010 Bari, Italy

来源：

JOURNAL OF VIROLOGICAL METHODS | 1998年 / 73卷 / 02期

关键词：

canine parvovirus; antigenic variants; characterization;

D O I：

10.1016/S0166-0934(98)00055-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

28 isolates of canine parvovirus type-2 (CPV-2) were obtained from dogs with hemorrhagic gastroenteritis in Italy. The antigenic structure of CPV-2 isolates was characterized, using four discriminating monoclonal antibodies. In addition, four vaccinal strains were examined. Similar to reports from Australia and the United Kingdom, a much higher prevalence of CPV-2a (25/28 isolates) was observed than the other variant type, CPV-2b (3/28 isolates). DNA fragments (2.2 kbp) of representative strains of CPV-2, CPV-2a and CPV-2b were amplified by the polymerase chain reaction (PCR) and the products were digested by the restriction enzymes (RE) RsaI, HpaII, HindIII and PvuII. The RsaI enzyme allows the differentiation of CPV-2 from CPV-2a and CPV-2b. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：197 / 200

页数：4

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