Isolation and characterization of a metsulfuron-methyl degrading bacterium Methylopila sp S113

被引：48

作者：

Huang, Xing ^{[1
]}

He, Jian ^{[1
]}

Sun, Jiquan ^{[1
]}

Pan, Jijie ^{[1
]}

Sun, Xiaofei ^{[1
]}

Li, Shunpeng ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Life Sci, Minist Agr, Key Lab Microbiol Engn Agr Environm, Nanjing 210095, Peoples R China

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2007年 / 60卷 / 03期

关键词：

sulfonylurea herbicides; metsulfuron-methyl; biodegradation; Methylopila sp; SULFONYLUREA HERBICIDES; SOIL; CHLORSULFURON; DEGRADATION; PERSISTENCE; STRAIN; BIODEGRADATION; MODE;

D O I：

10.1016/j.ibiod.2007.02.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The bacterium S113, capable of degrading metsulfuron-methyl, was isolated from metsulfuron-methyl-treated soil. The isolate was identified as Methylopila sp. according to its phenotypic features and 16S rDNA phylogenetic analysis. This strain could utilize metsulfuron-methyl as the sole carbon or nitrogen source. More than 97% of the 50 mgl(-1) initially added metsulfuron-methyl was depleted after 72h when a culture was inoculated with 104 cells l(-1) of strain S113. This strain could also degrade bensulfuron-methyl, thifensulfuron-methyl and ethametsulfuron-methyl. Cell-free extract of S113 was able to metabolize metsulfuron-methyl and other sulfonylurea herbicides. The metsulfuron-methyl degrading enzyme(s) was(ere) constitutively expressed and was(ere) not induced by metsulfuron-methyl. Inoculation of strain S113 into soil was found to promote the removal of metsulfuron-metbyl in soil. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：152 / 158

页数：7

共 28 条

[1] ACCELERATED PARATHION DEGRADATION IN SOIL INOCULATED WITH ACCLIMATED BACTERIA UNDER FIELD CONDITIONS [J].

BARLES, RW ;

DAUGHTON, CG ;

HSIEH, DPH .

ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1979, 8 (06) :647-660

[2] A REVIEW OF THE ACTIVITY, FATE AND MODE OF ACTION OF SULFONYLUREA HERBICIDES [J].

BLAIR, AM ;

MARTIN, TD .

PESTICIDE SCIENCE, 1988, 22 (03) :195-219

[3] Biodegradation of chlorsulfuron and metsulfuron-methyl by Aspergillus niger in laboratory conditions [J].

Boschin, G ;

D'Agostina, A ;

Arnoldi, A ;

Marotta, E ;

Zanardini, E ;

Negri, M ;

Valle, A ;

Sorlini, C .

JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 2003, 38 (06) :737-746

[4] Determination of sulfonylurea degradation products in soil by liquid chromatography-ultraviolet detection followed by confirmatory liquid chromatography-tandem mass spectrometry [J].

Bossi, R ;

Vejrup, K ;

Jacobsen, CS .

JOURNAL OF CHROMATOGRAPHY A, 1999, 855 (02) :575-582

[5] MODE OF ACTION, CROP SELECTIVITY, AND SOIL RELATIONS OF THE SULFONYLUREA HERBICIDES [J].

BROWN, HM .

PESTICIDE SCIENCE, 1990, 29 (03) :263-281

[6] Methylopila capsulata gen. nov., sp. nov., a novel non-pigmented aerobic facultatively methylotrophic bacterium [J].

Doronina, NV ;

Trotsenko, YA ;

Krausova, VI ;

Boulygina, ES ;

Tourova, TP .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 :1313-1321

[7] ISOLATION AND CHARACTERIZATION OF METHANOBREVIBACTER-ORALIS SP-NOV [J].

FERRARI, A ;

BRUSA, T ;

RUTILI, A ;

CANZI, E ;

BIAVATI, B .

CURRENT MICROBIOLOGY, 1994, 29 (01) :7-12

[8] The influence of chlorsulfuron and metsulfuron methyl on root growth and on the ultrastructure of root tips of germinating maize seeds [J].

Flaburiari, A ;

Kristen, U .

PLANT AND SOIL, 1996, 180 (01) :19-28

[9]

Holt G.J., 1994, Bergey's Manual of Determinative Bacteriology

[10] PERSISTENCE OF METSULFURON-METHYL IN 2 SOILS [J].

ISMAIL, BS ;

LEE, HJ .

JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 1995, 30 (04) :485-497

← 1 2 3 →