Distribution of catechol-O-methyltransferase expression in human central nervous system

被引：101

作者：

Jiang, H ^{[1
]}

Ho, SL ^{[1
]}

Xie, T ^{[1
]}

Young, LP ^{[1
]}

机构：

[1] Univ Hong Kong, Queen Mary Hosp, Dept Med, Div Neurol, Hong Kong, Peoples R China

来源：

NEUROREPORT | 1998年 / 9卷 / 12期

关键词：

central nervous system; distribution; isoforms; human COMT; mRNA;

D O I：

10.1097/00001756-199808240-00033

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

CATECHOL-O-METHYLTRANSFERASE (COMT, EC 2.1.1.6) is a ubiquitous enzyme crucial to catechol metabolism. Two isoforms exist in the human central nervous system (CNS) and they are encoded by two transcripts (1.3 and 1.5 kb) in most human tissues. Using two alpha-P-32-labeled probes, we found only the 1.5 kb transcript in all 16 regions of the human CNS using commercially available Northern blots. Spinal cord had the highest and amygdala had the lowest levels of expression. The other CNS regions shared a similar level of expression. The distributions of COMT gene expression relative to whole brain between both probes were significantly correlated. Our study shows that the expression of the 1.5 kb transcript is crucial for COMT activity in all regions of the human CNS. (C) 1998 Lippincott Williams & Wilkins.

引用

页码：2861 / 2864

页数：4

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