Simulation and experimental validation of micro polymerase chain reaction chips

被引：60

作者：

Lin, YC ^{[1
]}

Yang, CC ^{[1
]}

Huang, MY ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 70101, Taiwan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2000年 / 71卷 / 1-2期

关键词：

PCR; DNA; FEA; microfabrication;

D O I：

10.1016/S0925-4005(00)00595-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This study used finite element analysis to simulate the temperature characteristics of a micro polymerase chain reaction (PCR) chip. The micro-PCR chip was fabricated on a silicon wafer and Pyrex glass using photolithography, wet etching, and anodic bonding methods. The main goal of this study was to analyze the temperature uniformity and distribution of the micro-PCR chip, the temperature distribution of the DNA sample, and the transient temperature difference between the heater and DNA sample. The finite element analysis results were also confirmed by one-dimensional theoretic analysis. The simulation results were used to improve the thermal cycling time of a rapid micro-PCR system, consisting of a rapid thermal cycling system and a micro-PCR chip. The improved thermal cycles of the rapid mu PCR system were verified using serum samples from patients with chronic hepatitis C. The hepatitis C virus (HCV) amplicon of the rapid mu PCR system was analyzed by slab gel electrophoresis with DNA marker separation in parallel. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：127 / 133

页数：7

共 14 条

[1] Transient liquid crystal thermometry of microfabricated PCR vessel arrays [J].

Chaudhari, AM ;

Woudenberg, TM ;

Albin, M ;

Goodson, KE .

JOURNAL OF MICROELECTROMECHANICAL SYSTEMS, 1998, 7 (04) :345-355

[2] Chip PCR .2. Investigation of different PCR amplification systems in microfabricated silicon-glass chips [J].

Cheng, J ;

Shoffner, MA ;

Hvichia, GE ;

Kricka, LJ ;

Wilding, P .

NUCLEIC ACIDS RESEARCH, 1996, 24 (02) :380-385

[3]

DESHPANDE M, 1999, MSM1999

[4] DNA SEQUENCING WITH THERMUS-AQUATICUS DNA-POLYMERASE AND DIRECT SEQUENCING OF POLYMERASE CHAIN REACTION-AMPLIFIED DNA [J].

INNIS, MA ;

MYAMBO, KB ;

GELFAND, DH ;

BROW, MAD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) :9436-9440

[5]

KONDO S, 1999, P 10 INT C SOL STAT, P1718

[6] PLANAR CHIPS TECHNOLOGY FOR MINIATURIZATION AND INTEGRATION OF SEPARATION TECHNIQUES INTO MONITORING SYSTEMS - CAPILLARY ELECTROPHORESIS ON A CHIP [J].

MANZ, A ;

HARRISON, DJ ;

VERPOORTE, EMJ ;

FETTINGER, JC ;

PAULUS, A ;

LUDI, H ;

WIDMER, HM .

JOURNAL OF CHROMATOGRAPHY, 1992, 593 (1-2) :253-258

[7] HIGH-SPEED POLYMERASE CHAIN-REACTION IN CONSTANT FLOW [J].

NAKANO, H ;

MATSUDA, K ;

YOHDA, M ;

NAGAMUNE, T ;

ENDO, I ;

YAMANE, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (02) :349-352

[8]

Northrup M. A., 1995, P 8 INT C SOL STAT S, P764

[9] A miniature analytical instrument for nucleic acids based on micromachined silicon reaction chambers [J].

Northrup, MA ;

Benett, B ;

Hadley, D ;

Landre, P ;

Lehew, S ;

Richards, J ;

Stratton, P .

ANALYTICAL CHEMISTRY, 1998, 70 (05) :918-922

[10]

Northrup MA., 1993, TRANSDUCERS, P924

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