Nuclear magnetic resonance spectroscopic based studies of the metabolism of black tea polyphenols in humans

被引：88

作者：

Daykin, CA ^{[1
]}

Van Duynhoven, JPM ^{[1
]}

Groenewegen, A ^{[1
]}

Dachtler, M ^{[1
]}

Van Amelsvoort, JMM ^{[1
]}

Mulder, TPJ ^{[1
]}

机构：

[1] Unilever Res Labs, NL-3133 AT Vlaardingen, Netherlands

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2005年 / 53卷 / 05期

关键词：

metabonomics; NMR spectroscopy; urine; black tea; polyphenols; phenolic acids; principal components analysis; metabolism; bioavailability;

D O I：

10.1021/jf048439o

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Epidemiological studies indicate that a high intake of flavonoids is associated with an improved health status. Tea is one of the most abundant sources of flavonoids in the human diet. The bioavailability and biotransformation of tea flavonoids are, however, not clearly understood. The aim of the present study was to investigate the metabolism of black tea via a nonspecific screening method. H-1 nuclear magnetic resonance (NMR) spectroscopy was used to obtain nonselective profiles of urine samples collected from three human volunteers before and after a single dose of black tea. The complex spectroscopic profiles were interpreted with the use of pattern recognition techniques. Hippuric acid was confirmed as the major urinary black tea metabolite. One previously unknown metabolite was detected and identified as 1,3-dihydroxyphenyl-2-O-sulfate (sulfate conjugate of pyrogallol) using HPLC directly coupled to mass spectrometry and H-1 NMR spectroscopy. This study shows that NMR-pattern recognition studies can be used for the discovery of unknown flavonoid metabolites in humans.

引用

页码：1428 / 1434

页数：7

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