A method to score micronuclei in vivo using cytochalasin B-induced cytokinesis block

被引：8

作者：

Devi, PU ^{[1
]}

Rao, BSS ^{[1
]}

Kamath, R ^{[1
]}

机构：

[1] Kasturba Med Coll & Hosp, Dept Radiobiol, Manipal 576119, Karnataka, India

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1998年 / 401卷 / 1-2期

关键词：

mouse tumor; cytochalasin B; in vivo cytokinesis block; micronucleus;

D O I：

10.1016/S0027-5107(97)00227-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present paper describes an in vivo micronucleus assay using Cytochalasin B (CyB). Mice bearing three different rumours, fibrosarcoma (Swiss albino mice), B16 F1 melanoma(C57 BL) and Ehrlich ascites carcinoma (Swiss albino mice), were injected with repeated doses of CyB at different time intervals and binucleate cells were scored at 24, 36, 48, 60 and 72 h after CyB injection. Tt was found that three doses of 3 + 2 + 2 mg/kg CyB administered intraperitoneally (i.p.) at 12-h intervals effectively blocked cytokinesis. The maximum number of binucleated cells (BNC) was scored at 60 h after the last CyB dose. This dose schedule was also effective in scoring micronuclei in BNC after irradiation. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：33 / 37

页数：5

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