Electronic-energy migration and molecular rotation within bichromophoric macromolecules .1. Test of a model using bis(9-anthrylmethylphosphonate) bisteroid

被引:32
作者
Johansson, LBA [1 ]
Bergstrom, F [1 ]
Edman, P [1 ]
Grechishnikova, IV [1 ]
Molotkovsky, JG [1 ]
机构
[1] RUSSIAN ACAD SCI,MM SHEMYAKIN & OVCHINNIKOV INST BIOORGAN CHEM,MOSCOW,RUSSIA
来源
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS | 1996年 / 92卷 / 09期
关键词
D O I
10.1039/ft9969201563
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
We report a model for determining the rate of energy migration, within pairs of donor (D) molecules from the fluorescence anisotropy. The D molecules within each pair reside in an anisotropic environment, and undergo rotational motions, similar to what could be the case in a protein molecule. To test the model experimentally, we have synthesized mono- and bis(9-anthrylmethylphosphonate) bisteroid molecules. A procedure is presented for extracting the rate of energy transfer, as well as the D-D distance from the fluorescence anisotropy. The rate of energy migration obtained from experiments, omega approximate to 3.5 x 10(8) s(-1), agrees very well with that predicted. The distance 23.7 +/- 2 Angstrom between the anthracenes and the mutual angle of 131 +/- 3 degrees between their orientational distributions, obtained at different temperatures, are in excellent agreement with independently determined values.
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页码:1563 / 1567
页数:5
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