A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1

被引：240

作者：

de Berardinis, Veronique ^{[1
,2
]}

Vallenet, David ^{[1
,2
]}

Castelli, Vanina ^{[1
,2
]}

Besnard, Marielle ^{[1
,2
]}

Pinet, Agnes ^{[1
,2
]}

Cruaud, Corinne ^{[1
]}

Samair, Sumitta ^{[1
]}

Lechaplais, Christophe ^{[1
,2
]}

Gyapay, Gabor ^{[1
,2
]}

Richez, Celine ^{[1
]}

Durot, Maxime ^{[1
,2
]}

Kreimeyer, Annett ^{[1
,2
]}

Le Fevre, Francois ^{[1
,2
]}

Schaechter, Vincent ^{[1
,2
]}

Pezo, Valerie ^{[1
]}

Doering, Volker ^{[3
]}

Scarpelli, Claude ^{[1
]}

Medigue, Claudine ^{[1
,2
]}

Cohen, Georges N. ^{[1
]}

Marliere, Philippe ^{[3
]}

Salanoubat, Marcel ^{[1
,2
]}

Weissenbach, Jean ^{[1
,2
]}

机构：

[1] Genoscope Inst Genom CEA, F-91057 Evry, France

[2] Univ Evry, CNRS, UMR 8030, Evry, France

[3] Isthmus, Evry, France

来源：

MOLECULAR SYSTEMS BIOLOGY | 2008年 / 4卷 / 1期

关键词：

Acinetobacter baylyi ADP1; deletion mutants; essential genes; growth phenotype; metabolism; systems biology;

D O I：

10.1038/msb.2008.10

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have constructed a collection of single-gene deletion mutants for all dispensable genes of the soil bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were obtained, whereas 499 (16%) were not, and are therefore candidate essential genes for life on minimal medium. This essentiality data set is 88% consistent with the Escherichia coli data set inferred from the Keio mutant collection profiled for growth on minimal medium, while 80% of the orthologous genes described as essential in Pseudomonas aeruginosa are also essential in ADP1. Several strategies were undertaken to investigate ADP1 metabolism by (1) searching for discrepancies between our essentiality data and current metabolic knowledge, (2) comparing this essentiality data set to those from other organisms, (3) systematic phenotyping of the mutant collection on a variety of carbon sources (quinate, 2-3 butanediol, glucose, etc.). This collection provides a new resource for the study of gene function by forward and reverse genetic approaches and constitutes a robust experimental data source for systems biology approaches.

引用

页数：15

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