The CD271 expression could be alone for establisher phenotypic marker in Bone Marrow derived mesenchymal stem cells

被引：30

作者：

Flores-Torales, Edgardo ^{[1
,2
]}

Orozco-Barocio, Arturo ^{[2
]}

Gonzalez-Ramella, Oscar R. ^{[1
,3
]}

Carrasco-Yalan, Antonio ^{[1
]}

Gazarian, Karlen ^{[4
]}

Cuneo-Pareto, Silvio ^{[1
]}

机构：

[1] Sangre de Cordon SA de CV, Guadalajara 44600, Jalisco, Mexico

[2] Univ Guadalajara, Dept Biol Celular & Mol, Ctr Univ Ciencias Biol & Agr, Guadalajara, Jalisco, Mexico

[3] Univ Guadalajara, Dept Fisiol, Ctr Univ Ciencias Salud, Guadalajara, Jalisco, Mexico

[4] Univ Nacl Autonoma Mexico, Dept Biol Mol & Biotecnol, Inst Invest Biomed, Mexico City 04510, DF, Mexico

来源：

FOLIA HISTOCHEMICA ET CYTOBIOLOGICA | 2010年 / 48卷 / 04期

关键词：

mesenchymal stem cells; CD271; bone marrow; surface markers; STROMAL CELLS; INTERNATIONAL-SOCIETY; PROGENITOR CELLS; MSC; EXPANSION; EXHIBIT;

D O I：

10.2478/v10042-010-0063-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Mesenchymal stem cells (MSCs) are of great interest for their potential use in cellular therapies. To define the population more precisely, diverse surface markers have been used. We propose here to use CD271 as the sole marker for MSCs in fresh bone marrow. We compared CD271(+) populations to the presence or absence of five defined markers for MSCs: CD90(+), CD105(+), CD45(-), CD34(-) and CD79. The correlations between markers were evaluated and analyzed with a Pearson's correlation test. We found that the average percentage of cells expressing the combination of markers CD90(+), CD105(+), CD45-, CD34(-) and CD79(-) was 0.54%, and that the average percentage average of CD271(+) cells was 0.53%. The results were significant (p<0.05). The exclusive use of CD271 as a marker for MSCs from fresh samples of bone marrow appears to be highly selective. Using CD271 as the sole identification marker for MSCs could reduce costs and accelerate the process of identifying MSCs for the field of cellular therapy.

引用

页码：682 / 686

页数：5

共 30 条

[1]

Cotransplantation of human mesenchymal stem cells enhances human myelopoiesis and megakaryocytopoiesis in NOD/SCID mice [J].