Purification and cDNA cloning of Luxuriosin, a novel antibacterial peptide with Kunitz domain from the Longicorn Beetle, Acalolepta luxuriosa

被引：11

作者：

Ueda, K

Saito, A

Imamura, M

Miura, N

Atsumi, S

Tabunoki, H

Watanabe, A

Kitami, M

Sato, R

机构：

[1] Tokyo Univ Agr & Technol, Grad Sch Bioappl & Syst Engn, Koganei, Tokyo 1848588, Japan

[2] Natl Inst Anim Hlth, Prion Dis Res Ctr, Tsukuba, Ibaraki 3050856, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2005年 / 1722卷 / 01期

关键词：

antimicrobial peptide; antibacterial peptide; antifungal peptide; Kunitz; Acalolepta luxuriosa; insect immunity;

D O I：

10.1016/j.bbagen.2004.11.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have purified a novel antibacterial peptide from the hemolymph of the coleopteran insect Acalolepta luxuriosa, of the family Qerambyocidae, and named it luxuriosin. This peptide showed growth-inhibitory activity against Micrococcus luteus and germination-and/or growtb-inhibitory activity against the conidia from rice blast fungus, Magnaporthe grisea. The amino acid sequence determined by cDNA cloning identified luxuriosin as a peptide of 88 amino acids with a theoretical molecular weight of 10,368.34, containing a Kunitz domain. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：36 / 42

页数：7

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