FET based biosensor for estimation of the effect of Ca2+ and Mg2+ on the immobilised butyrylcholinesterase

被引：5

作者：

Khomutov, SM ^{[1
]}

Sidorov, IA ^{[1
]}

Reshetilov, AN ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Biochem & Physiol Microorganisms, Pushchino 142292, Moscow Region, Russia

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1998年 / 48卷 / 1-3期

关键词：

FET; butyrylcholinesterase; activation by icons; Ca2+; Mg2+; positive cooperativity;

D O I：

10.1016/S0925-4005(98)00086-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The activity of immobilised butyrylcholinesterase (BChE) was determined potentiometrically by field-effect-transistor (FET), using butyrylthiocholine iodide (BTCh) as a substrate. The enzyme activity increased in the presence of Ca2+ and Mg2+. Computation of experimental data showed: (1) the increase of enzyme and substrate affinity in the presence of Mg2+; and (2) the increase of the rate of formation of hydrolysis products from the enzyme-substrate complex in the presence of Ca2+ and Mg2+ Positive cooperativity was observed for Mg2+, the Hill coefficient increased from 1.23 to 2.37 with the increase of Mg2+ concentration from 0.1 to 10 mM. For Ca2+ the coefficient was about 1.0 independently from the concentration. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：467 / 470

页数：4

共 9 条

[1] ANTICHOLINESTERASE ACTIVITY MEASUREMENT BY A CHOLINE BIOSENSOR - APPLICATION IN WATER ANALYSIS [J].