Determination of moxifloxacin in growth media by high-performance liquid chromatography

A direct injection high-performance liquid chromatographic method with column switching has been developed to determine moxifloxacin in Mueller-Hinton broth. A LiChrocart 4-4 pre-column filled with a LiChrospher 100 RP 18.5 mum and a 150x4.6 mm I.D, column packed with a Supelcozil ABZ+ Plus were used and led to a retention time of 5.70 min. Fluorescence detection allowed one to reach a quantification limit of 0.05 mug/ml with a 100-mul sample size. The standard curves were linear from 0.05 to 3.2 mug/ml. Intra- and inter-day imprecisions within the linearity range were less than or equal to4.76 and less than or equal to5.75%, respectively. The mean relative errors for the same day and the day-to-day inaccuracies ranged from -2.93 to +4.50% and from -1.10 to +6.00%, respectively. The method was demonstrated to be useful for pharmacokinetic-pharmacodynamic studies of moxifloxacin in an in vitro model. (C) 2001 Elsevier Science B.V. All rights reserved.