Effect of serum metabolites of Pueraria lobata in rats on peripheral nerve regeneration:: In Vitro and in Vivo studies

被引：16

作者：

Chen, Hsien-Tung ^{[1
]}

Yao, Chun-Hsu ^{[2
]}

Chao, Pei-Dawn Lee ^{[3
]}

Hou, Yu-Chi ^{[4
]}

Chiang, Hsiu-Mei ^{[5
]}

Hsieh, Chang-Chi ^{[6
]}

Ke, Cherng-Jyh ^{[7
]}

Chen, Yueh-Sheng ^{[1
,8
]}

机构：

[1] China Med Univ, Grad Inst Chinese Med Sci, Lab Biomat, Taichung, Taiwan

[2] Cent Taiwan Univ Sci & Technol, Inst Biomed Engn & Mat Sci, Taichung, Taiwan

[3] China Med Univ, Sch Pharm, Taichung, Taiwan

[4] China Med Univ, Sch Chinese Med, Taichung, Taiwan

[5] China Med Univ, Dept Cosmet, Taichung, Taiwan

[6] China Med Univ, Grad Inst Integrat Chinese & Western Med, Taichung, Taiwan

[7] China Med Univ, Dept Med Radiol Technol, Taichung, Taiwan

[8] China Med Univ, Dept Med Radiol Technol, Taichung, Taiwan

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS | 2008年 / 84B卷 / 01期

关键词：

Pueraria lobata; peripheral nerve regeneration; PC12; cell; silicone rubber; serum metabolite;

D O I：

10.1002/jbm.b.30868

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 [生物医学工程];

摘要：

This study provides in vitro and in vivo evaluation of rat serum metabolites of the Pueraria lobata (SMP) on peripheral nerve regeneration. In the in vitro study, we found that the SNIP caused a marked enhancement of the nerve growth factor (NGF)-mediated neurite outgrowth and the expression of synapsin I from PC12 cells. In the in vivo study, silicone rubber chambers filled with the SNIP were used to bridge a 10-mm sciatic nerve defect in rats. At the conclusion of 8 weeks, animals from the groups treated with the SMP had a relatively more mature structure with larger mean values of myelinated axon number, endoneurial area, and total nerve area when compared with those in the controls receiving the saline only. These results suggest that the serum metabolites of Pueraria lobata can be a potential nerve growth-promoting factor. (c) 2007 Wiley Periodicals, Inc.

引用

页码：256 / 262

页数：7

共 26 条

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