Neuroprotective effects of chlorogenic acid on scopolamine-induced amnesia via anti-acetylcholinesterase and anti-oxidative activities in mice

Chlorogenic acid is a major polyphenolic component of many plants and beverages and is particularly abundant in coffee We evaluated the neuroprotective effects of chlorogenic acid on learning and memory impairment induced by scopolamine (0 5 mg/kg i p) a muscarinic antagonist using the Y-maze passive avoidance and Morris water maze tests The chlorogenic acid significantly improved the impairment of short-term or working memory induced by scopolamine in the Y-maze test, and significantly reversed cognitive impairments in mice as measured by the passive avoidance test In addition chlorogenic acid decreased escape latencies in the Morris water maze test In a probe trial session chlorogenic acid increased the latency time in the target quadrant in a dose dependent manner Ex vivo chlorogenic acid inhibited acetylcholinesterase activity in the hippocampus and frontal cortex Chlorogenic acid also decreased malondialdehyde levels in the hippocampus and frontal cortex In vitro chlorogenic acid was found to inhibit acetylcholinesterase activity (IC50 = 98 17 mu g/ml) and free radical scavenging activity (IC50 = 3 09 mu g/ml) in a dose-dependent manner These results indicate that chlorogenic acid may exert anti-amnesic activity via inhibition of acetylcholinesterase and malondialdehyde in the hippocampus and frontal cortex (C) 2010 Elsevier B V All rights reserved