Emergence of TCR α/β V(D)J recombination and transcription during ontogeny of inbred mouse strains

被引：8

作者：

Junta, CM

Passos, GAS ^{[1
]}

机构：

[1] Univ Sao Paulo, GIM, Dept Genet, Fac Med Ribeirao Preto, BR-14040900 Ribeirao Preto, SP, Brazil

[2] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Morfol, BR-14049 Ribeirao Preto, SP, Brazil

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1998年 / 187卷 / 1-2期

基金：

巴西圣保罗研究基金会;

关键词：

T-cell receptor genes; V(D)J recombination; TCR alpha/beta genes transcription; T-cell differentiation; thymus ontogeny;

D O I：

10.1023/A:1006807021251

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The maturation of T-cells depends on V(D)J recombination at the TCR alpha/beta and gamma/delta loci that occurs in the thymus during fetal development. Due to the essential role of the TCR beta in thymocyte differentiation, it is important to define with precision the temporal emergence of the TCR beta rearrangement and its expression in normal non-manipulated mouse strains. We studied the onset of the V(D)J recombination and transcription of the TCR V alpha 3 and V beta 11 genes during ontogeny in Balb-c, C57B1/6 and CBA inbred mouse strains. Our data show differences in the emergence of recombination in both TCR alpha and beta loci among strains. The transcriptions of these loci followed respective recombinations and we detected an early germline transcript before TCR beta locus recombination in the CBA strain.

引用

页码：67 / 72

页数：6

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