Catalpol Promotes the Proliferation and Differentiation of Osteoblasts Induced by High Glucose by Inhibiting KDM7A

被引：13

作者：

Cheng, Jian ^{[1
,2
]}

Xu, Hai-yan ^{[3
]}

Liu, Ming-ming ^{[4
]}

Cai, Jian-ping ^{[2
]}

Wang, Lei ^{[2
]}

Hua, Zhen ^{[2
]}

Wu, Xiao-dong ^{[1
]}

Huo, Wei-ling ^{[1
]}

Lv, Nan-ning ^{[4
]}

机构：

[1] Nanjing Univ Chinese Med, Dept Orthoped, Xuzhou Cent Hosp, Xuzhou 221009, Jiangsu, Peoples R China

[2] Nanjing Univ Chinese Med, Inst Traumatol & Orthoped, Nanjing 210029, Jiangsu, Peoples R China

[3] Xuzhou Med Univ, Dept Human Anat, Xuzhou 221004, Jiangsu, Peoples R China

[4] Lianyungang Second Peoples Hosp, Dept Orthoped Surg, 41 Hailian East Rd, Lianyungang 222023, Jiangsu, Peoples R China

来源：

DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY | 2020年 / 13卷

关键词：

catalpol; KDM7A; proliferation; differentiation; osteoblasts; high glucose; HISTONE; FAMILY;

D O I：

10.2147/DMSO.S246433

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Introduction: The protective effect of catalpol on diabetic osteoporosis (DOP) and its mechanism remain unclear. This study aimed to explore whether catalpol enhanced the proliferation and differentiation of MC3T3 cells induced by high glucose by inhibiting the expression of KDM7A. Methods: MC3T3 cells were induced by high glucose (HG) and treated with different concentrations of catalpol. The proliferation and mineralization abilities of MC3T3-E1 cells were determined by CCK-8 assay and Alizarin Red Staining, respectively. The expression of differentiation-related osteogenic proteins, KDM7A and related proteins of Wnt/beta-catenin signaling pathway was analyzed by Western blot analysis. The alkaline phosphatase (ALP) activity was detected by ALP assay kits. Results: MC3T3-E1 cells induced by high glucose showed decreased proliferation and mineralization abilities and decreased ALP activity, which were all reversed by the treatment of catalpol. High glucose induction inhibited the expression of KDM7A, Total-beta-catenin, Nuclear-beta-catenin and p-GSK3 beta, which was reversed by the treatment of catalpol. And KDM7A interference up-regulated the expression of Total-beta-catenin, Nuclear-beta-catenin and p-GSK3 beta, which was down-regulated by KDM7A overexpression. Furthermore, the proliferation and mineralization abilities and ALP activity were improved when treated with KDM7A interference and decreased when treated with KDM7A overexpression. However, SKL2001 could improve the proliferation and mineralization abilities and ALP activity of MC3T3-E1 cells. Discussion: Catalpol promotes the proliferation and differentiation of osteoblasts induced by high glucose by regulating the Wnt/beta-catenin signaling pathway through KDM7A.

引用

页码：705 / 712

页数：8

共 26 条

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