Quantification of reactive oxygen species generated by alveolar macrophages using lucigenin-enhanced chemiluminescence - Methodical aspects

被引：12

作者：

Brehm, M ^{[1
]}

Schiller, E ^{[1
]}

Zeller, WJ ^{[1
]}

机构：

[1] GERMAN CANC RES CTR,DEPT 0420,D-69009 HEIDELBERG,GERMANY

来源：

TOXICOLOGY LETTERS | 1996年 / 87卷 / 2-3期

关键词：

alveolar macrophages; lucigenin-enhanced chemiluminescence; quartz; reactive oxygen species; superoxide radical;

D O I：

10.1016/0378-4274(96)03768-X

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Alveolar macrophages lavaged from bovine lungs using Ca2+- and Mg2+-deprived saline containing EGTA for calcium chelation were cultivated in RPMI-1640 medium. The generation of reactive oxygen species (ROS) was assessed by determination of lucigenin-enhanced chemiluminescence (LUC-CL) using a LB 9505 C Biolumat. Monitoring of LUC-CL depends on various methodical parameters: besides medium constituents, the method of cell harvesting and the time-schedule of the protocol appear to be essential parameters that influence ROS-generation. In addition, the cell number (cell density) as well as the ratio of cell number to particle mass influence the amount of ROS-generation. Following exposure of cells to micronized quartz, we observed a dose-related increase in the generation of ROS.

引用

页码：131 / 138

页数：8

共 21 条

[1]

ALLEN RC, 1982, CHEM BIOL GENERATION, P309

[2] COMPARATIVE BIOCHEMICAL AND CYTOCHEMICAL STUDIES ON SUPEROXIDE AND PEROXIDE IN MOUSE MACROPHAGES [J].

BADWEY, JA ;

ROBINSON, JM ;

LAZDINS, JK ;

BRIGGS, RT ;

KARNOVSKY, MJ ;

KARNOVSKY, ML .

JOURNAL OF CELLULAR PHYSIOLOGY, 1983, 115 (02) :208-216

[3]

BERTHOLD F, 1987, CHEMILUMINESCENCE, V1, P49

[4] A COMPARATIVE STUDY OF GLYCOPROTEINS FROM SURFACE OF CONTROL AND ROUS SARCOMA VIRUS TRANSFORMED HAMSTER CELLS [J].

BUCK, CA ;

GLICK, MC ;

WARREN, L .

BIOCHEMISTRY, 1970, 9 (23) :4567-&

[5]

CZOP JK, 1982, AM REV RESPIR DIS, V125, P607

[6]

Elstner EF., 1990, Der Sauerstoff: Biochemie, Biologie

[7]

Gutteridge J. M. C., 1989, FREE RADICALS BIOL M

[8]

Halliwell B., 1991, AM J MED, V91, P14, DOI DOI 10.1016/0002-9343(91)90279-7

[9] FREE-RADICALS AS MEDIATORS OF TISSUE-INJURY AND DISEASE [J].

KEHRER, JP .

CRITICAL REVIEWS IN TOXICOLOGY, 1993, 23 (01) :21-48

[10] TOXICITY OF METALLIC-IONS AND OXIDES TO RABBIT ALVEOLAR MACROPHAGES [J].

LABEDZKA, M ;

GULYAS, H ;

SCHMIDT, N ;

GERCKEN, G .

ENVIRONMENTAL RESEARCH, 1989, 48 (02) :255-274

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