Measles virus protein-specific IgM, IgA, and IgG subclass responses during the acute and convalescent phase of infection

被引:27
作者
El Mubarak, HS
Ibrahim, SA
Vos, HW
Mukhtar, MM
Mustafa, OA
Wild, TF
Osterhaus, ADME
de Swart, RL
机构
[1] Erasmus MC, Dept Virol, NL-3000 DR Rotterdam, Netherlands
[2] Univ Khartoum, Inst Endem Dis, Khartoum, Sudan
[3] CERVI, INERM U404 Immunite & Vaccinat, Lyon, France
关键词
fusion protein; haemagglutinin; nucleoprotein; antibody; isotype; avidity;
D O I
10.1002/jmv.10553
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The availability of new generation serological assays allowed re-evaluation of the antibody response to measles virus. IgM, IgA, total IgG, and IgG subclass responses were studied to the three major immunogenic measles virus proteins: the fusion protein (F), haemagglutinin (H), and nucleoprotein (N). Plasma samples were obtained from clinically diagnosed measles cases (n = 146) in Khartoum (Sudan) within a week after onset of the rash. Convalescent phase samples were collected from 32 of 117 laboratory-confirmed measles cases at different time points after onset of rash. Glycoprotein-specific IgM, IgG, and IgA antibody levels correlated well to the N-specific response. For IgG and IgA, responses to F were higher than to H. IgA antibody levels were undetectable in about one third of the laboratory-confirmed cases during the acute phase, but positive in all patients tested 1-4 weeks after infection. IgM levels declined rapidly and were lost 3-6 months after infection. IgA levels declined slowly during the first year but did not return to background levels during the subsequent 2 years. IgG avidity maturation was detected during a 3-6 month period after infection. The predominant IgG subclasses during the acute phase were IgG(1) and IgG(3). The latter was lost in the convalescent phase, while the IgG(4) isotype showed a slight rise afterwards. Interestingly, acute phase IgG(3) and IgA responses were associated, and were only detected in samples with high IgG. This study provides a comprehensive perspective on the antibody response to wildtype measles virus infection. (C) 2004 Wiley-Liss, Inc.
引用
收藏
页码:290 / 298
页数:9
相关论文
共 32 条
[1]  
AALBERSE RC, 1983, J IMMUNOL, V130, P722
[2]  
[Anonymous], 2001, FIELDS VIROLOGY
[3]   ESTABLISHMENT AND CHARACTERIZATION OF MURINE CELLS CONSTITUTIVELY EXPRESSING THE FUSION, NUCLEOPROTEIN AND MATRIX PROTEINS OF MEASLES-VIRUS [J].
BEAUVERGER, P ;
BUCKLAND, R ;
WILD, F .
JOURNAL OF VIROLOGICAL METHODS, 1993, 44 (2-3) :199-210
[4]   Neutralizing B cell response in measles [J].
Bouche, FB ;
Ertl, OT ;
Muller, CP .
VIRAL IMMUNOLOGY, 2002, 15 (03) :451-471
[5]   Evaluation of hemagglutinin protein-specific immunoglobulin M for diagnosis of measles by an enzyme-linked immunosorbent assay based on recombinant protein produced in a high-efficiency mammalian expression system [J].
Bouche, FB ;
Brons, NHC ;
Houard, S ;
Schneider, F ;
Muller, CP .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (12) :3509-3513
[6]   Prevention of measles in Sudan: a prospective study vaccination, diagnosis and epidemiology [J].
de Swart, RL ;
El Mubarak, HS ;
Vos, HW ;
Mustafa, OM ;
Abdallah, A ;
Groen, J ;
Mukhtar, MM ;
Zijlstra, EE ;
El Hassan, AM ;
Wild, TF ;
Ibrahim, SA ;
Osterhaus, ADME .
VACCINE, 2001, 19 (17-19) :2254-2257
[7]   Measles virus fusion protein- and hemagglutinin-transfected cell lines are a sensitive tool for the detection of specific antibodies by a FACS-measured immunofluorescence assay [J].
de Swart, RL ;
Vos, HW ;
UytdeHaag, FGCM ;
Osterhaus, ADME ;
van Binnendijk, RS .
JOURNAL OF VIROLOGICAL METHODS, 1998, 71 (01) :35-44
[8]  
EHRNST A, 1978, J IMMUNOL, V121, P1206
[9]  
El Mubarak HS, 2000, J CLIN MICROBIOL, V38, P987
[10]   Genetic characterization of wild-type measles viruses circulating in suburban Khartoum, 1997-2000 [J].
El Mubarak, HS ;
van de Bildt, MWG ;
Mustafa, OA ;
Vos, HW ;
Mukhtar, MM ;
Ibrahim, SA ;
Andeweg, AC ;
El Hassan, AM ;
Osterhaus, ADME ;
de Swart, RL .
JOURNAL OF GENERAL VIROLOGY, 2002, 83 :1437-1443