Effects of α-adrenoreceptor antagonists on apoptosis and proliferation of pancreatic cancer cells in vitro

被引:36
作者
Shen, Su-Gang [1 ]
Zhang, Dong [1 ]
Hu, Heng-Tong [1 ]
Li, Jun-Hui [1 ]
Wang, Zheng [1 ]
Ma, Qing-Yong [1 ]
机构
[1] Xi An Jiao Tong Univ, Dept Hepatobiliary & Pancreas Surg, Affiliated Hosp 1, Coll Med, Xian 710061, Shaanxi Prov, Peoples R China
关键词
alpha-adrenoreceptor; pancreatic cancer; yohimbine; urapidil hydrochloride;
D O I
10.3748/wjg.14.2358
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To discuss the expression of alpha-adrenoreceptors in pancreatic cancer cell lines PC-2 and PC-3 and the effects of alpha 1- and alpha 2-adrenoreceptor antagonists, yohimbine and urapidil hydrochloride, on the cell lines in vitro. METHODS: We cultured the human ductal pancreatic adenocarcinoma cell lines PC-2 and PC-3 and analyzed the mRNA expression of alpha 1- and alpha 2-adrenergic receptors by reverse transcription polymerase chain reaction (RT-PCR). The effects of yohimbine and urapidil hydrochloride on cell proliferation were assessed by 3-(4,5-dimethylthiasol-2-yl)2,4,-diphenyltetrazolium bromide (MTT) assay. Apoptosis was detected using the terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick-end labeling (TUNEL) assay and flow cytometry (FCM). RESULTS: PC-2 expressed mRNA in alpha 1- and alpha 2-adrenoreceptors. MTT assays showed that urapidil hydrochloride had no effect on PC-3 cell lines. However, exposure to urapidil hydrochloride increased DNA synthesis in PC-2 cell lines as compared to the control group. PC-2 cell lines were sensitive to both drugs. The proliferation of the 2 cell lines was inhibited by yohimbine. Cell proliferation was inhibited by yohimbine via apoptosis induction. CONCLUSION: The expression of alpha 1- and alpha 2-adrenoreceptors; is different in PC-2 and PC-3 cell lines, which might be indicative of their different functions. The alpha 2-adrenoceptor antagonist, yohimbine, can inhibit the proliferation of both cell lines and induce their apoptosis, suggesting that yohimbine can be used as an anticancer drug for apoptosis of PC-2 and PC-3 cells. (c) 2008 WJG. All rights reserved.
引用
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页码:2358 / 2363
页数:6
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