Sperm Methylation Profiles Reveal Features of Epigenetic Inheritance and Evolution in Primates

被引：300

作者：

Molaro, Antoine ^{[1
]}

Hodges, Emily ^{[1
]}

Fang, Fang ^{[2
]}

Song, Qiang ^{[2
]}

McCombie, W. Richard ^{[1
]}

Hannon, Gregory J. ^{[1
]}

Smith, Andrew D. ^{[2
]}

机构：

[1] Cold Spring Harbor Lab, Howard Hughes Med Inst, Cold Spring Harbor, NY 11724 USA

[2] Univ So Calif, Los Angeles, CA 90089 USA

来源：

CELL | 2011年 / 146卷 / 06期

关键词：

DE-NOVO METHYLATION; DNA METHYLATION; CYTOSINE METHYLATION; HUMAN GENOME; CPG ISLANDS; VERTEBRATE GENOMES; BINDING-PROTEIN; SVA ELEMENTS; STEM-CELLS; GERM-CELLS;

D O I：

10.1016/j.cell.2011.08.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During germ cell and preimplantation development, mammalian cells undergo nearly complete reprogramming of DNA methylation patterns. We profiled the methylomes of human and chimp sperm as a basis for comparison to methylation patterns of ESCs. Although the majority of promoters escape methylation in both ESCs and sperm, the corresponding hypomethylated regions show substantial structural differences. Repeat elements are heavily methylated in both germ and somatic cells; however, retrotransposons from several subfamilies evade methylation more effectively during male germ cell development, whereas other subfamilies show the opposite trend. Comparing methylomes of human and chimp sperm revealed a subset of differentially methylated promoters and strikingly divergent methylation in retrotransposon subfamilies, with an evolutionary impact that is apparent in the underlying genomic sequence. Thus, the features that determine DNA methylation patterns differ between male germ cells and somatic cells, and elements of these features have diverged between humans and chimpanzees.

引用

页码：1028 / 1040

页数：13

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