Mediated, amperometric biosensor for glucose-6-phosphate monitoring based on entrapped glucose-6-phosphate dehydrogenase, Mg2+ ions, tetracyanoquinodimethane, and nicotinamide adenine dinucleotide phosphate in carbon paste

被引：20

作者：

Bassi, AS ^{[1
]}

Tang, DQ ^{[1
]}

Bergougnou, MA ^{[1
]}

机构：

[1] Univ Western Ontario, Fac Engn Sci, Dept Chem & Biochem Engn, London, ON N6A 5B9, Canada

来源：

ANALYTICAL BIOCHEMISTRY | 1999年 / 268卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1006/abio.1998.3082

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, an amperometric carbon paste biosensor is developed for glucose-6-phosphate (G6P) monitoring which is based on entrapped Mg2+ ions, G6P dehydrogenase, NADP(+) polyethylenimine (PEI) and the electroactive mediator, tetracyanoquinodimethane (TCNQ), The calibration line had a slope of 1.55 x 10(-5) A.M-1 with a correlation coefficient of 0.9965. The limit of detection (de fined as three times the standard deviation of the response of the electrode to blank phosphate buffer injections (noise)) of the G6P biosensor was 5.0 x 10(-5) M. The application of this biosensor for monitoring G6P in human blood using the standard addition method is also demonstrated. A two-parameter empirical equation which adequately describes the deactivation of the biosensor steady-state response with time is also proposed. (C) 1999 Academic Press.

引用

页码：223 / 228

页数：6

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