Differential epigenetic regulation of Aiolos expression in human tumoral cell lines and primary cells

被引：6

作者：

Duhamel, Marianne ^{[1
,2
]}

Navarro, Pablo ^{[3
]}

Cario-Andre, Muriel ^{[4
]}

Billot, Katy ^{[1
,2
]}

Arrouss, Issam ^{[1
,2
]}

Rebollo, Angelita ^{[1
,2
]}

机构：

[1] Hop La Pitie Salpetriere, INSERM, U543, F-75013 Paris, France

[2] Univ Paris 06, Paris, France

[3] Inst Pasteur, Mouse Mol Genet Unit, F-75015 Paris, France

[4] Univ V Segalen, INSERM, U876, F-33076 Bordeaux, France

来源：

FEBS LETTERS | 2008年 / 582卷 / 03期

关键词：

Aiolos; CpG islands; DNA methylation; histone modifications; transcriptional regulation;

D O I：

10.1016/j.febslet.2008.01.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

In order to investigate the epigenetic component of Aiolos regulation, we analyzed the methylation status of its 5' CpG island in relation to historic modifications. Inhibition of CpG methylation restores Aiolos expression, as well as euchromatin-associated markers, in U937 and 1106 mel cell lines. DNA methylation and low levels of euchromatin-associated signatures are observed in U937 and 1106 mel cell lines, while the opposite characterizes Daudi, Jurkat, T and B cells. CpG methylation is not necessary to repress transcription in monocytes and melanocytes where silencing mechanism involves heterochromatin-associated signature. We show that DNA methylation directs Aiolos silencing and chromatin status in tumor cell lines, while in primary cells is mainly regulated by histone modifications. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：457 / 467

页数：11

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