Molecular authentication of Panax species

被引：112

作者：

Ngan, F

Shaw, P

But, P

Wang, J ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Dept Biochem, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Dept Biol, Hong Kong, Peoples R China

[3] Chinese Univ Hong Kong, Chinese Med Mat Res Ctr, Hong Kong, Peoples R China

来源：

PHYTOCHEMISTRY | 1999年 / 50卷 / 05期

关键词：

Panax ginseng; P-quinquefolius; araliaceae; ginseng; traditional Chinese medicinal materials; polymerase chain reaction; authentication; restriction fragment length polymorphism; ribosomal DNA;

D O I：

10.1016/S0031-9422(98)00606-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using conserved plant sequences as primers, the DNA sequences in the ribosomal ITS1-5.8S-ITS2 region have been amplified and determined for six Panax species, P. ginseng C. A. Mey. (Oriental ginseng), P. quinquefolius L. (American ginseng), P. notoginseng (Burkill) F. H. Chen (Sanchi), P. japonicus C. A. Mey. (Japanese ginseng), P. trifolius L. and P. major Ting, as well as two Common adulterants of ginseng, Mirabilis jalapa L. and Phytolacca acinosa Roxb. An authentication procedure based upon the restriction fragment length polymorphism (RFLP) in the region is able to differentiate between P, ginseng and P, quinquefolius, and to discriminate the ginsengs from the two common poisonous adulterants. Broader application of this approach to authenticate other morphologically similar Chinese medicinal materials is rationalised. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：787 / 791

页数：5

共 20 条

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