Protease-activated receptor genes are clustered on 5q13

被引:8
作者
Dupérat, VG
Jacquelin, B
Boisseau, P
Arveiler, B
Nurden, AT
机构
[1] Hop Cardiol, CNRS, UMR 5533, F-33604 Pessac, France
[2] Univ Bordeaux 2, Lab Pathol Mol & Therapie Gen, F-33076 Bordeaux, France
关键词
D O I
10.1182/blood.V92.1.25.413k41_25_31
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The serine protease, thrombin, is both a potent agonist for platelet aggregation and a mitogen inducing the proliferation of other cell types. Many cellular responses to thrombin are mediated by a G-protein-coupled thrombin receptor (protease-activated receptor-1, PAR-1). This represents the prototype of a new family of proteolytically cleaved receptors that includes PAR-2 and the recently identified PAR-3, Like PAR-1, PAR-3 is a potential thrombin receptor. Their similar gene structure, mechanism of activation, and colocalization to 5q13 raises the question of a common evolutionary origin and of their belonging to a clustered gene family. Construction of a physical map of the 5q13 region by pulsed-field gel electrophoresis (PFGE) has allowed us to identify six potential CpG islands and to establish a linkage of the PAR genes, Southern blot analysis showed that they were in a cluster on a 560-kb Asc I fragment, in the order PAR-2, PAR-1, and PAR-3. PAR-1 and PAR-2 genes were contained within the identical 240-kb Not I fragment, thus confirming a tight linkage between them. The localization of other CpG islands suggested that more PAR-family genes may be present. (C) 1998 by The American Society of Hematology.
引用
收藏
页码:25 / 31
页数:7
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