Synthesis and activity of bivalent FKBP12 ligands for the regulated dimerization of proteins

被引：59

作者：

Keenan, T ^{[1
]}

Yaeger, DR ^{[1
]}

Courage, NL ^{[1
]}

Rollins, CT ^{[1
]}

Pavone, ME ^{[1
]}

Rivera, VM ^{[1
]}

Yang, W ^{[1
]}

Guo, T ^{[1
]}

Amara, JF ^{[1
]}

Clackson, T ^{[1
]}

Gilman, M ^{[1
]}

Holt, DA ^{[1
]}

机构：

[1] Ariad Gene Therapeut, Cambridge, MA 02139 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 1998年 / 6卷 / 08期

关键词：

D O I：

10.1016/S0968-0896(98)00125-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The total synthesis and in vitro activities of a series of chemical inducers of dimerization (CIDs) is described. The use of small-molecule CIDs to control the dimerization of engineered FKBP12-containing fusion proteins has been demonstrated to have broad utility in biological research as well as potential medical applications in gene and cell therapies. The facility and flexibility of preparation make this new class of wholly synthetic compounds exceptionally versatile tools for the study of intracellular signaling events mediated by protein-protein interactions or protein localization. While some congeners possess potency comparable to or better than the first generation natural product-derived CID, FK1012, structure-activity relationships are complex and underscore the need for application-specific compound optimizations. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.

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页码：1309 / 1335

页数：27

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