Melatonin prevents acute kidney injury in severely burned rats via the activation of SIRT1

被引：86

作者：

Bai, Xiao-Zhi ^{[1
]}

He, Ting ^{[1
]}

Gao, Jian-Xin ^{[1
]}

Liu, Yang ^{[1
]}

Liu, Jia-Qi ^{[1
]}

Han, Shi-Chao ^{[1
]}

Li, Yan ^{[1
]}

Shi, Ji-Hong ^{[1
]}

Han, Jun-Tao ^{[1
]}

Tao, Ke ^{[1
]}

Xie, Song-Tao ^{[1
]}

Wang, Hong-Tao ^{[1
]}

Hu, Da-Hai ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Burns & Cutaneous Surg, 127 Changle West Rd, Xian 710032, Shaanxi, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

中国国家自然科学基金;

关键词：

MESENCHYMAL STEM-CELL; INDUCED RENAL INJURY; OXIDATIVE STRESS; INFLAMMATORY RESPONSES; ISCHEMIA-REPERFUSION; ORGAN DYSFUNCTION; PROTECTS; APOPTOSIS; TRANSCRIPTION; ANTIOXIDANT;

D O I：

10.1038/srep32199

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Acute kidney injury (AKI) is a common complication after severe burns. Melatonin has been reported to protect against multiple organ injuries by increasing the expression of SIRT1, a silent information regulator that regulates stress responses, inflammation, cellular senescence and apoptosis. This study aimed to investigate the protective effects of melatonin on renal tissues of burned rats and the role of SIRT1 involving the effects. Rat severely burned model was established, with or without the administration of melatonin and SIRT1 inhibitor. The renal function and histological manifestations were determined to evaluate the severity of kidney injury. The levels of acetylated-p53 (Ac-p53), acetylated-p65 (Ac-p65), NF-kappa B, acetylated-forkhead box O1 (Ac-FoxO1), Bcl-2 and Bax were analyzed to study the underlying mechanisms. Our results suggested that severe burns could induce acute kidney injury, which could be partially reversed by melatonin. Melatonin attenuated oxidative stress, inflammation and apoptosis accompanied by the increased expression of SIRT1. The protective effects of melatonin were abrogated by the inhibition of SIRT1. In conclusion, we demonstrate that melatonin improves severe burn-induced AKI via the activation of SIRT1 signaling.

引用

页数：13

共 57 条

[1]

Melatonin ameliorates metabolic risk factors, modulates apoptotic proteins, and protects the rat heart against diabetes-induced apoptosis [J].