cAMP modulates macrophage development by suppressing M-CSF-induced MAPKs activation

被引：19

作者：

Zhu, Ning ^{[1
,2
]}

Cui, Jian ^{[2
]}

Qiao, Chunxia ^{[2
]}

Li, Yan ^{[1
,2
]}

Ma, Yuanfang ^{[1
]}

Zhang, Jiyan ^{[2
]}

Shen, Beifen ^{[1
,2
]}

机构：

[1] Henan Univ, Cellular & Mol Immunol Lab, Kaifeng 475001, Peoples R China

[2] Inst Basic Med Sci, Dept Mol Immunol, Beijing 100850, Peoples R China

来源：

CELLULAR & MOLECULAR IMMUNOLOGY | 2008年 / 5卷 / 02期

基金：

中国国家自然科学基金;

关键词：

macrophage development; M-CSF; cAMP; MAPKs;

D O I：

10.1038/cmi.2008.19

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

M-CSF is a key cytokine in macrophage development by inducing MAPKs activation, and cAMP can inhibit MAPKs activation induced by inflammatory stimuli. To explore the effects of cAMP on M-CSF-induced MAPKs activation and on macrophage development, the model of bone marrow-derived murine macrophages (BMMs) was used. The effects of cAMP on M-CSF-induced MAPKs activation were analyzed by Western blotting assay, and the effects of cAMP on CD14 and F4/80 expression during macrophage development were examined by FACS analysis. Macrophage morphology showed the successful establishment of the model of macrophage development. Western blotting assay revealed that M-CSF activated ERK, JNK and p38 in both mature and immature macrophages, and cAMP inhibited M-CSF-induced ERK, JNK and p38 activation in a time-dependent manner. FACS analysis revealed that macrophage development was impaired with cAMP pretreatment. In conclusion, cAMP modulates macrophage development by suppressing M-CSF-induced MAPKs activation.

引用

页码：153 / 157

页数：5

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