TWIK-2, a new weak inward rectifying member of the tandem pore domain potassium channel family

被引:114
作者
Chavez, RA
Gray, AT
Zhao, BB
Kindler, CH
Mazurek, MJ
Mehta, Y
Forsayeth, JR
Yost, CS
机构
[1] Elan Pharmaceut, Menlo Park, CA 94025 USA
[2] Univ Calif San Francisco, Sch Med, Dept Anesthesia & Perioperat Care, San Francisco, CA 94143 USA
关键词
D O I
10.1074/jbc.274.12.7887
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Potassium channels are found in all mammalian cell types, and they perform many distinct functions in both excitable and non-excitable cells. These functions are subserved by several different families of potassium channels distinguishable by primary sequence features as well as by physiological characteristics. Of these families, the tandem pore domain potassium channels are a new and distinct class, primarily distinguished by the presence of two pore-forming domains within a single polypeptide chain. We have cloned a new member of this family, TWIK-2, from a human brain cDNA library. Primary sequence analysis of TWIK-2 shows that it is most closely related to TWIK-1, especially in the pore-forming domains. Northern blot analysis reveals the expression of TWIK-2 in all human tissues assayed except skeletal muscle. Human TWIK-2 expressed heterologously in Xenopus oocytes is a non-inactivating weak inward rectifier with channel properties similar to TWIK-1, Pharmacologically, TWIK-2 channels are distinct from TWIK-1 channels in their response to quinidine, quinine, and barium. TWIK-2 is inhibited by intracellular, but not extracellular, acidification. This new clone reveals the existence of a subfamily in the tandem pore domain potassium channel family with weak inward rectification properties.
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页码:7887 / 7892
页数:6
相关论文
共 28 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]   A Ba2+-insensitive K+ conductance in the basolateral membrane of rabbit cortical thick ascending limb cells [J].
Di Stefano, A ;
Greger, R ;
Desfleurs, E ;
de Rouffignac, C ;
Wittner, M .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 1998, 8 (1-2) :89-105
[3]   THE INWARD RECTIFIER POTASSIUM CHANNEL FAMILY [J].
DOUPNIK, CA ;
DAVIDSON, N ;
LESTER, HA .
CURRENT OPINION IN NEUROBIOLOGY, 1995, 5 (03) :268-277
[4]   The structure of the potassium channel:: Molecular basis of K+ conduction and selectivity [J].
Doyle, DA ;
Cabral, JM ;
Pfuetzner, RA ;
Kuo, AL ;
Gulbis, JM ;
Cohen, SL ;
Chait, BT ;
MacKinnon, R .
SCIENCE, 1998, 280 (5360) :69-77
[5]   TASK, a human background K+ channel to sense external pH variations near physiological pH [J].
Duprat, F ;
Lesage, F ;
Fink, M ;
Reyes, R ;
Heurteaux, C ;
Lazdunski, M .
EMBO JOURNAL, 1997, 16 (17) :5464-5471
[6]   SPERMINE AND SPERMIDINE AS GATING MOLECULES FOR INWARD RECTIFIER K+ CHANNELS [J].
FICKER, E ;
TAGLIALATELA, M ;
WIBLE, BA ;
HENLEY, CM ;
BROWN, AM .
SCIENCE, 1994, 266 (5187) :1068-1072
[7]   A neuronal two P domain K+ channel stimulated by arachidonic acid and polyunsaturated fatty acids [J].
Fink, M ;
Lesage, F ;
Duprat, F ;
Heurteaux, C ;
Reyes, R ;
Fosset, M ;
Lazdunski, M .
EMBO JOURNAL, 1998, 17 (12) :3297-3308
[8]   Cloning, functional expression and brain localization of a novel unconventional outward rectifier K+ channel [J].
Fink, M ;
Duprat, F ;
Lesage, F ;
Reyes, R ;
Romey, G ;
Heurteaux, C ;
Lazdunski, M .
EMBO JOURNAL, 1996, 15 (24) :6854-6862
[9]  
GUILLEMARE E, 1995, MOL PHARMACOL, V47, P588
[10]   A novel subunit for Shal K+ channels radically alters activation and inactivation [J].
Jegla, T ;
Salkoff, L .
JOURNAL OF NEUROSCIENCE, 1997, 17 (01) :32-44