Comparative analysis of gas production profiles obtained with buffalo and sheep ruminal fluid as the source of inoculum

被引:35
作者
Calabrò, S
López, S
Piccolo, V
Dijkstra, J
Dhanoa, MS
France, J
机构
[1] Univ Leon, Dept Anim Prod, E-24071 Leon, Spain
[2] Univ Naples Federico II, Dipartimento Sci Zootecn & Ispez Alimenti, I-80137 Naples, Italy
[3] Wageningen Univ, WIAS Anim Nutr Grp, NL-6709 PG Wageningen, Netherlands
[4] Inst Grassland & Environm Res, Aberystwyth SY23 3EB, Dyfed, Wales
[5] Univ Guelph, Ctr Nutr Modelling, Dept Anim & Poultry Sci, Guelph, ON N1G 2W1, Canada
关键词
gas production; rumen; in vitro fermentation kinetics; buffalo; sheep;
D O I
10.1016/j.anifeedsci.2005.04.039
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
An in vitro study was conducted to investigate effects of inoculum source (i.e., sheep versus buffalo rumen fluid) on gas production profiles, and to evaluate the suitability of various mathematical equations to fit the profiles and provide accurate values of degradation attributes. Incubations were completed using ruminal fluid obtained from sheep or buffalos fed the same diet (600g/kg grass hay and 400g/kg concentrate). Kinetics of fermentation of five feeds commonly fed to ruminants, being maize silage, grass silage, wheat straw, barley grain and a mixed hay, were studied with a gas production technique using an automated pressure evaluation system (APES). Ruminal fermentation characteristics (i.e., substrate disappearance, pH and VFA production) were determined after 120 h of incubation. Five mathematical functions (i.e., exponential, France, Gompertz, logistic, Morgan) were fitted to the experimental data to estimate rate and extent of feed degradation. Model comparison was based on goodness-of-fit assessed from analysis of residual variance and Akaike's information criterion. The logistic and Morgan functions were best overall, although the goodness-of-fit attained with all models was considered acceptable. Except for the Morgan, there were small differences among models in values derived for extent of degradation. There were differences between sources of inoculum in gas production measured at intermediate times (i.e., gas volumes with buffalo rumen fluid of 133 and 164 ml/g organic matter (OM) at 24 h for grass hay and silage, respectively, were smaller than those with sheep rumen fluid of 182 and 208 ml/g OM), but not at earlier or later incubation times. As a result of this trend, shorter half times, faster fermentation rates and higher extents of degradation occurred when feeds were incubated in sheep (estimated OM degradability was 0.308 and 0.402 g/g OM for grass hay and silage, respectively) compared with buffalo rumen fluid (0.246 and 0.330 g/g OM). Differences were larger for more fibrous substrates (i.e., grass silage, straw and hay) and negligible for barley grain. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:51 / 65
页数:15
相关论文
共 45 条
[1]  
ADESOGAN AT, 1998, OCCASIONAL PUBLICATI, V22, P215
[2]  
AOAC, 1999, OFF METH AN ASS OFF
[3]   Solid and fluid passage rate in buffalo, cattle and sheep fed diets with different forage to concentrate ratios [J].
Bartocci, S ;
Amici, A ;
Verna, M ;
Terramoccia, S ;
Martillotti, F .
LIVESTOCK PRODUCTION SCIENCE, 1997, 52 (03) :201-208
[4]   Characteristics of foodstuffs and diets, and the quanti-qualitative milk parameters of Mediterranean buffaloes bred in Italy using the intensive system - An estimate of the nutritional requirements of buffalo herds lactating or dry [J].
Bartocci, S ;
Tripaldi, C ;
Terramoccia, S .
LIVESTOCK PRODUCTION SCIENCE, 2002, 77 (01) :45-58
[5]   MODELING GAS-PRODUCTION KINETICS OF GRASS SILAGES INCUBATED WITH BUFFERED RUMINAL FLUID [J].
BEUVINK, JMW ;
KOGUT, J .
JOURNAL OF ANIMAL SCIENCE, 1993, 71 (04) :1041-1046
[6]   In vitro gas production: A technique revisited [J].
Blummel, M ;
Makkar, HPS ;
Becker, K .
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION, 1997, 77 (01) :24-34
[7]  
Burnham K. P., 2002, MODEL SELECTION MULT, DOI [10.1007/b97636, DOI 10.1007/B97636]
[8]   A comparison between buffalo (Bubalus bubalis) and cow (Bos taurus) rumen fluids in terms of the in vitro fermentation characteristics of three fibrous feedstuffs [J].
Calabrò, S ;
Williams, BA ;
Piccolo, V ;
Infascelli, F ;
Tamminga, S .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2004, 84 (07) :645-652
[9]   Influence of rumen fluid and substrate concentration on fermentation kinetics measured with a fully automated time related gas production apparatus [J].
Cone, JW ;
vanGelder, AH ;
Visscher, GJW ;
Oudshoorn, L .
ANIMAL FEED SCIENCE AND TECHNOLOGY, 1996, 61 (1-4) :113-128
[10]   An automated system for measuring gas production from forages inoculated with rumen fluid and its use in determining the effect of enzymes on grass silage [J].
Davies, ZS ;
Mason, D ;
Brooks, AE ;
Griffith, GW ;
Merry, RJ ;
Theodorou, MK .
ANIMAL FEED SCIENCE AND TECHNOLOGY, 2000, 83 (3-4) :205-221