Evaluation of BACTEC MGIT 960 and BACTEC 460TB systems for recovery of mycobacteria from clinical specimens of a university hospital with low incidence of tuberculosis

被引:32
作者
Leitritz, L [1 ]
Schubert, S [1 ]
Bücherl, B [1 ]
Masch, A [1 ]
Heesemann, J [1 ]
Roggenkamp, A [1 ]
机构
[1] Univ Munich, Max Von Pettenkofer Inst Hyg & Med Microbiol, D-80336 Munich, Germany
关键词
D O I
10.1128/JCM.39.10.3764-3767.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Clinical samples obtained over a period of 8 months (n = 2,624) were processed in parallel with the BACTEC 460TB system, with the MGIT 960 system, and in Lowenstein-Jensen (Lj) medium, resulting in the recovery of 127 mycobacteria. Recovery rates in combinations of the BACTEC 460TB or MGIT 960 system with LJ were, respectively, 94.7 and 94.7% for Mycobacterium tuberculosis complex (n = 57) and 91.4 and 70.0% for nontuberculous mycobacteria (n = 70). Contamination rates, elevated in the MGIT 960 system, were associated with patients (cystic fibrosis) and type of material but not with transport time. Detection time was reduced in the MGIT 960 system.
引用
收藏
页码:3764 / 3767
页数:4
相关论文
共 16 条
[1]   NONTUBERCULOUS MYCOBACTERIAL DISEASE IN ADULT CYSTIC-FIBROSIS PATIENTS [J].
AITKEN, ML ;
BURKE, W ;
MCDONALD, G ;
WALLIS, C ;
RAMSEY, B ;
NOLAN, C .
CHEST, 1993, 103 (04) :1096-1099
[2]  
Alcaide F, 2000, J CLIN MICROBIOL, V38, P398
[3]  
Dean A.G, 1995, EPI INFO VERSION 6 W
[4]   Multicenter evaluation of the BACTEC MGIT 960 system for recovery of mycobacteria [J].
Hanna, BA ;
Ebrahimzadeh, A ;
Elliott, LB ;
Morgan, MA ;
Novak, SM ;
Rusch-Gerdes, S ;
Acio, M ;
Dunbar, DF ;
Holmes, TM ;
Rexer, CH ;
Savthyakumar, C ;
Vannier, AM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (03) :748-752
[5]   Evaluation of the BACTEC™ MGIT™ 960 system for the recovery of mycobacteria [J].
Kanchana, MV ;
Cheke, D ;
Natyshak, I ;
Connor, B ;
Warner, A ;
Martin, T .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2000, 37 (01) :31-36
[6]   NONTUBERCULOUS MYCOBACTERIA IN ADULT PATIENTS WITH CYSTIC-FIBROSIS [J].
KILBY, JM ;
GILLIGAN, PH ;
YANKASKAS, JR ;
HIGHSMITH, WE ;
EDWARDS, LJ ;
KNOWLES, MR .
CHEST, 1992, 102 (01) :70-75
[7]   GENOTYPIC IDENTIFICATION OF MYCOBACTERIA BY NUCLEIC-ACID SEQUENCE DETERMINATION - REPORT OF A 2-YEAR EXPERIENCE IN A CLINICAL LABORATORY [J].
KIRSCHNER, P ;
SPRINGER, B ;
VOGEL, U ;
MEIER, A ;
WREDE, A ;
KIEKENBECK, M ;
BANGE, FC ;
BOTTGER, EC .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (11) :2882-2889
[8]   COMPARISON OF A RADIOMETRIC METHOD (BACTEC) AND CONVENTIONAL CULTURE MEDIA FOR RECOVERY OF MYCOBACTERIA FROM SMEAR-NEGATIVE SPECIMENS [J].
MORGAN, MA ;
HORSTMEIER, CD ;
DEYOUNG, DR ;
ROBERTS, GD .
JOURNAL OF CLINICAL MICROBIOLOGY, 1983, 18 (02) :384-388
[9]  
Nolte Frederick S., 1995, P400
[10]   Comparison of the Bactec 460TB system and the Bactec MGIT 960 system in recovery of mycobacteria from clinical specimens [J].
Pinheiro, MD ;
Ribeiro, MM .
CLINICAL MICROBIOLOGY AND INFECTION, 2000, 6 (03) :171-173