Quantifying on- and off-target genome editing

被引：113

作者：

Hendel, Ayal ^{[1
]}

Fine, Eli J. ^{[2
,3
]}

Bao, Gang ^{[2
,3
]}

Porteus, Matthew H. ^{[1
]}

机构：

[1] Stanford Univ, Dept Pediat, Stanford, CA 94305 USA

[2] Georgia Inst Technol, Dept Biomed Engn, Atlanta, GA 30332 USA

[3] Emory Univ, Atlanta, GA 30332 USA

来源：

TRENDS IN BIOTECHNOLOGY | 2015年 / 33卷 / 02期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

gene editing; gene targeting; homologous recombination; nonhomologous end-joining; ZFNs; TALENs; CRISPR/Cas9; RNA-guided endonucleases; ZINC-FINGER NUCLEASES; ANALYSIS REVEALS; WIDE ANALYSIS; HUMAN-CELLS; IN-VITRO; CAS9; CLEAVAGE; SPECIFICITY; ENDONUCLEASE; TALENS;

D O I：

10.1016/j.tibtech.2014.12.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genome editing with engineered nucleases is a rapidly growing field thanks to transformative technologies that allow researchers to precisely alter genomes for numerous applications including basic research, biotechnology, and human gene therapy. While the ability to make precise and controlled changes at specified sites throughout the genome has grown tremendously in recent years, we still lack a comprehensive and standardized battery of assays for measuring the different genome editing outcomes created at endogenous genomic loci. Here we review the existing assays for quantifying on- and off-target genome editing and describe their utility in advancing the technology. We also highlight unmet assay needs for quantifying on- and off-target genome editing outcomes and discuss their importance for the genome editing field.

引用

页码：132 / 140

页数：9

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