DNA aptamers as potential anti-HIV agents

被引：64

作者：

Chou, SH ^{[1
]}

Chin, KH

Wang, AHJ

机构：

[1] Natl Chung Hsing Univ, Inst Biochem, Taichung 40227, Taiwan

[2] Acad Sinica, Inst Biol Chem, Nankang, Taiwan

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 2005年 / 30卷 / 05期

关键词：

D O I：

10.1016/j.tibs.2005.03.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Guanine (G)-rich DNA sequences can adopt stable G-quadruplex structures by G-tetrad hydrogen-bonding and hydrophobic stacking. Recently, it has been shown that a DNA sequence forms an aptamer (termed 93del) and adopts a novel dimeric quadruplex folding topology in K+ solution. This aptamer exhibits anti-HIV1 integrase activity in the nanomolar range in vitro. A docking-based model of the 93del-integrase complex positions the DNA aptamer within a channel of the tetrameric integrase. This mutual fitting blocks several catalytic amino acid residues that are essential for integrase function, and accounts for the anti-HIV1 activity of the 93del aptamer.

引用

页码：231 / 234

页数：4

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