Production and characterization of Phanerochaete chrysosporium lignin peroxidases for pulp bleaching

被引:10
作者
de Carvalho, MEA
Monteiro, MC
Bon, EPS
Sant'Anna, GL
机构
[1] Fac Engn Quim Lorena, Dept Biotecnol, BR-12600000 Lorena, SP, Brazil
[2] Univ Fed Rio de Janeiro, COPPE, BR-21945 Rio De Janeiro, Brazil
[3] Univ Fed Rio de Janeiro, Inst Quim, Rio De Janeiro, Brazil
关键词
Phanerochaete chrysosporium; lignin peroxidase; enzymatic prebleaching; eucalyptus kraft pulp; selectivity;
D O I
10.1007/BF02920206
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The production of lignin peroxidase from Phanerochaete chrysosporium was studied using immobilized mycelia in nylon-web cubes in semicontinuous fermentation using glucose pulses or ammonium tartrate pulses. Consistent enzyme production was achieved when glucose pulses were used, leading to an average activity of 253 U/L. The crude enzyme was added to eucalyptus kraft pulp before conventional and ECF bleaching sequences. Optimization of the enzymatic pretreatment led to the following operational conditions: enzyme load of 2 U/g of pulp, hydrogen peroxide addition rate of 10 ppm/h, and reaction time of 60 min. Pulp final characteristics were dependent on the chemical treatment sequence that followed enzymatic pretreatment. The chief advantage of enzymatic pretreatment was pulp viscosity preservation, which was observed in most of the experiments carried out with seven different chemical treatment sequences.
引用
收藏
页码:955 / 966
页数:12
相关论文
共 23 条
[1]   PASSIVE IMMOBILIZATION OF ASPERGILLUS-AWAMORI SPORES FOR SUBSEQUENT GLUCOAMYLASE PRODUCTION [J].
BON, E ;
WEBB, C .
ENZYME AND MICROBIAL TECHNOLOGY, 1989, 11 (08) :495-499
[2]  
CHARNEY J, 1947, J BIOL CHEM, V171, P501
[3]   PROTEASE-MEDIATED DEGRADATION OF LIGNIN PEROXIDASE IN LIQUID CULTURES OF PHANEROCHAETE-CHRYSOSPORIUM [J].
DOSORETZ, CG ;
DASS, SB ;
REDDY, CA ;
GRETHLEIN, HE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (11) :3429-3434
[4]   EFFECT OF ENVIRONMENTAL-CONDITIONS ON EXTRACELLULAR PROTEASE ACTIVITY IN LIGNOLYTIC CULTURES OF PHANEROCHAETE-CHRYSOSPORIUM [J].
DOSORETZ, CG ;
CHEN, HC ;
GRETHLEIN, HE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (02) :395-400
[5]  
ERIKSSON KE, 1985, TAPPI J, V68, P46
[6]  
ERIKSSON KEL, 1990, WOOD SCI TECHNOL, V24, P79, DOI 10.1007/BF00225309
[7]   ROLE OF VERATRYL ALCOHOL IN REGULATING LIGNINASE ACTIVITY IN PHANEROCHAETE-CHRYSOSPORIUM [J].
FAISON, BD ;
KIRK, TK ;
FARRELL, RL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1986, 52 (02) :251-254
[8]   PRODUCTION OF LIGNIN PEROXIDASE BY PHANEROCHAETE-CHRYSOSPORIUM IN A PACKED-BED BIOREACTOR OPERATED IN SEMICONTINUOUS MODE [J].
FEIJOO, G ;
DOSORETZ, C ;
LEMA, JM .
JOURNAL OF BIOTECHNOLOGY, 1995, 42 (03) :247-253
[9]   EVIDENCE FOR VERATRYL ALCOHOL AS A REDOX MEDIATOR IN LIGNIN PEROXIDASE-CATALYZED OXIDATION [J].
GOODWIN, DC ;
AUST, SD ;
GROVER, TA .
BIOCHEMISTRY, 1995, 34 (15) :5060-5065
[10]   EFFECTS OF FUNGAL AND ENZYMATIC TREATMENTS ON ISOLATED LIGNINS AND ON PULP BLEACHABILITY [J].
KANTELINEN, A ;
HORTLING, B ;
RANUA, M ;
VIIKARI, L .
HOLZFORSCHUNG, 1993, 47 (01) :29-35