Non-radioactive assay of natural killer cell-mediated cytotoxicity against cytomegalovirus-infected fibroblasts by DNA fragmentation ELISA

被引：4

作者：

Ito, M ^{[1
]}

Watanabe, M ^{[1
]}

Kamiya, H ^{[1
]}

Sakurai, M ^{[1
]}

机构：

[1] MIE NATL HOSP, DEPT PEDIAT, TSU, MIE, JAPAN

来源：

JOURNAL OF VIROLOGICAL METHODS | 1996年 / 56卷 / 01期

关键词：

natural killer cell; cytomegalovirus; DNA fragmentation; cytotoxic assay; ELISA;

D O I：

10.1016/0166-0934(95)01954-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell-mediated cytotoxicity against cytomegalovirus (CMV)-infected fibroblasts (FS-4 cells) was investigated by a non-radioactive assay, and by DNA fragmentation ELISA and LDH release assay and the assays were compared to the standard chromium release assay. Fragmentation of DNA and LDH activity were detected in the supernatant of CMV-infected FS-4 cells cultured with non-adherent peripheral blood mononuclear cells (PBMC). The DNA fragmentation ELISA was most sensitive to cytotoxicity against CMV-infected FS-4 cells and showed excellent correlation with the standard chromium release assay. DNA fragmentation of CMV-infected FS-4 cells by non-adherent PBMC was reduced markedly by treatment with anti-Leu 11b plus complement. Thus, the present DNA fragmentation ELISA is non-radioactive, highly sensitive and a useful method for detecting natural killer cell-mediated cytotoxicity against CMV-infected fibroblasts.

引用

页码：77 / 84

页数：8

共 25 条

[1] LYMPHOCYTE-TRIGGERED INTERNAL TARGET DISINTEGRATION [J].