IFNβ bioavailability in multiple sclerosis patients:: MxA versus antibody-detecting assays

被引：24

作者：

Capra, Ruggero

Sottini, Alessandra

Cordioli, Cinzia

Serana, Federico

Chiarini, Marco

Caimi, Luigi

Padovani, Alessandro

Bergamaschi, Roberto

Imberti, Luisa

机构：

[1] Sped Civil Brescia, Multiple Sclerosis Ctr, Dept Neurol, Brescia, Italy

[2] Sped Civil Brescia, Dept Diagnost, Biotechnol Lab, Brescia, Italy

[3] Univ Brescia, Dept Neurol, Brescia, Italy

[4] Neurol Inst C Mondino, Pavia, Italy

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2007年 / 189卷 / 1-2期

关键词：

interferons; multiple sclerosis; MxA; bioavailability; NAbs; BAbs;

D O I：

10.1016/j.jneuroim.2007.06.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Anti-IFN beta antibodies are related to IFN beta bioavailability loss in multiple sclerosis. We investigated the reliability of radioimmunoprecipitation and cytopathic assays in detecting binding (BAbs) and neutralizing (NAbs) antibodies and the correlation of these antibodies to MxA mRNA production. Eleven percent of IFN beta-treated patients showed a lack of MxA induction, with an inverse correlation between MxA mRNA and the presence of BAbs and NAbs. Some patients had contemporary MxA induction in the presence of high NAb titres, thus calling into question the reliability of cytopathic assay. Since anti-IFN beta antibodies well correlated with MxA induction loss, MxA assay is an appropriate test to determine IFN beta bioavailability. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：102 / 110

页数：9

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