The development of diurnal rhythmicity in fetal suprachiasmatic neurons as demonstrated by Fos immunohistochemistry

Using Fos immunohistochemistry as a marker of cellular activity, we have shown that neurons in the suprachiasmatic nucleus of fetal sheep are active by 35 days gestation. From at least 90 days gestation (term is 146 days), these neurons are more active during the day (12.00) than at night (03.00) when pregnant ewes are exposed to a 12-h light-dark cycle with lights on at 07.00. The day-night difference in Fos immunoreactivity persisted when the lighting schedule was extended by Sh to 03.00, although neurons were now more active at 03.00 than they were in fetuses maintained on the normal light-dark cycle. When ewes were maintained in constant light from 133 to 138 days, the day-night difference in Fos immunoreactivity in the fetal suprachiasmatic nucleus was abolished, suggesting that diurnal activity of the fetal suprachiasmatic nucleus is maintained by a signal related to the external lighting regime. In a twin pregnancy where one fetus was optically enucleated at 100 days gestation, the density of Fos-immunoreactive neurons in the suprachiasmatic nucleus during the day at 138 days was similar to the unoperated twin. This suggests that the effects of dim light in the uterus on the fetal retina do not account for the high level of Fos immunoreactivity in the suprachiasmatic nucleus in the daytime. We propose that a chemical messenger of maternal origin, possibly melatonin, suppresses the activity of fetal suprachiasmatic neurons during the night, and that fetal suprachiasmatic neurons have endogenous activity which is expressed fully during the daytime. Copyright (C) 1996 IBRO.