Determination of triterpene glycosides in sea cucumber (Stichopus japonicus) and its related products by high-performance liquid chromatography

被引：19

作者：

Dong, Ping ^{[1
]}

Xue, Chang-hu ^{[1
]}

Yu, Lin-fang ^{[1
]}

Xu, Jie ^{[1
]}

Chen, Shi-guo ^{[1
]}

机构：

[1] Ocean Univ China, Coll Food Sci & Technol, Qingdao 266003, Shandong, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2008年 / 56卷 / 13期

关键词：

sea cucumber; Stichopus japonicus; triterpene glycoside determination; 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization; high-performance liquid chromatography (HPLC);

D O I：

10.1021/jf800893r

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A creative and sensitive method has been developed. for the determination of triterpene glycosides concentrations in sea cucumber (Stichopus japonicus) and related products by using D-quinovose (6-deoxyglucose) as the measurement standard by reverse-phase high-performance liquid chromatography (HPLC) and variable-wavelength detection. D-quinovose, which is a unique monosaccharide in holostane triterpene glycosides, was liberated by acid hydrolysis and precolumn derivatized by 1-phenyl-3-methyl-5-pyrazolone (PMP). PMP-quinovose was analyzed by HPLC with 22% acetonitrile in 0.05 M KH2PO4 aquatic solution (pH 5.2) as mobile phase. The calibration curves Of D-quinovose were linear within the range of 6.56-164 mg/L (r(2) > 0.995). The contents of triterpene glycosides in various S. japonicus products were determined after appropriate pretreatment methods. The concentration of triterpene glycosides was calculated by the formula C = C-qui x alpha (alpha = 8.5). The result showed that this method was a simple, rapid, and stable method for the determination of triterpene glycosides in S. japonicus products.

引用

页码：4937 / 4942

页数：6

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