Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves: Falconiformes, Accipitridae)

被引：35

作者：

Hailer, F

Gautschi, B

Helander, B

机构：

[1] Uppsala Univ, Evolutionary Biol Ctr, Dept Evolutionary Biol, SE-75236 Uppsala, Sweden

[2] Ecogen GmbH, CH-8952 Zurich, Switzerland

[3] Swedish Museum Nat Hist, Contaminant Res Grp, SE-10405 Stockholm, Sweden

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 04期

关键词：

Accipitridae; conservation genetics; Haliaeetus albicilla; microsatellites; multiplex PCR;

D O I：

10.1111/j.1471-8286.2005.01122.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the development of 14 novel polymorphic microsatellite markers cloned from the White-tailed Sea Eagle, Haliaeetus albicilla, a formerly threatened raptor that has received much conservation attention throughout Eurasia. We also present a protocol for multiplex polymerase chain reaction (PCR) amplification of the loci. Among 40 unrelated H. albicilla individuals from southern Sweden, the markers produced two to eight alleles per locus, and average observed and expected heterozygosities were 0.463 and 0.468, respectively. We further present five microsatellite markers that appeared monomorphic in H. albicilla, but which may be of interest for use in other raptor species.

引用

页码：938 / 940

页数：3

共 11 条

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