Osteoprogenitor cells within skeletal muscle

被引:210
作者
Bosch, P
Musgrave, DS
Lee, JY
Cummins, J
Shuler, F
Ghivizzani, SC
Evans, C
Robbins, PD
Huard, J [1 ]
机构
[1] Childrens Hosp Pittsburgh, Dept Orthopaed Surg & Mol Genet & Biochem, Growth & Dev Lab, Pittsburgh, PA 15261 USA
[2] Univ Pittsburgh, Musculoskeletal Res Ctr, Dept Orthopaed Surg, Growth & Dev Lab, Pittsburgh, PA 15261 USA
[3] Univ Pittsburgh, Dept Orthopaed Surg, Ferguson Lab, Pittsburgh, PA 15260 USA
[4] Univ Pittsburgh, Dept Mol Genet & Biochem, Pittsburgh, PA 15260 USA
关键词
D O I
10.1002/jor.1100180613
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
The formation of ectopic bone within skeletal muscle is a widely observed phenomenon. However, the source of the osteoprogenitor cells responsible for ectopic bone formation remains unknown. This study was designed to test for osteogenic differentiation among cells isolated from skeletal muscle tissue. Different subpopulations of cells derived from an adult mouse skeletal muscle were tested for induction of alkaline phosphatase activity after exposure to bone morphogenetic protein-2 in vitro. A responsive subpopulation was identified, transduced with a retrovirus encoding for beta -galactosidase (Rv-lacZ) and an adenoviral construct encoding for one bone morphogenetic protein-2, and injected into the hindlimb of immune compromised (severe combined immunodeficient, or SCID) mice. The injected cells appeared to actively participate in the ectopic bone formation. The existence of lacZ-positive muscle-derived cells colocalized with osteocalcin-producing cells within lacunae of newly formed bone matrix suggests osteoblast and osteocyte differentiation. Although a specific cell was not isolated, these data support the contentions that osteoprogenitor cells reside within skeletal muscle and that muscle may represent a source other than bone marrow for the harvest of these cells.
引用
收藏
页码:933 / 944
页数:12
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共 70 条
  • [1] Acceleration of experimental endochondral ossification by biophysical stimulation of the progenitor cell pool
    Aaron, RK
    Ciombor, DM
    [J]. JOURNAL OF ORTHOPAEDIC RESEARCH, 1996, 14 (04) : 582 - 589
  • [2] Aikawa T, 1996, J BONE MINER RES, V11, P544
  • [3] Effect of rhBMP-2 on the osteogenic potential of bone marrow stromal cells from an osteogenesis imperfecta mouse (oim)
    Balk, ML
    Bray, J
    Day, C
    Epperly, M
    Greenberger, J
    Evans, CH
    Niyibizi, C
    [J]. BONE, 1997, 21 (01) : 7 - 15
  • [4] BONE-MARROW DERIVED STROMAL CELL-LINE EXPRESSING OSTEOBLASTIC PHENOTYPE INVITRO AND OSTEOGENIC CAPACITY INVIVO
    BENAYAHU, D
    KLETTER, Y
    ZIPORI, D
    WIENTROUB, S
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 1989, 140 (01) : 1 - 7
  • [5] DESMIN AND VIMENTIN IN REGENERATING MUSCLES
    BORNEMANN, A
    SCHMALBRUCH, H
    [J]. MUSCLE & NERVE, 1992, 15 (01) : 14 - 20
  • [6] BOYAN BD, 1992, CLIN ORTHOP RELAT R, P266
  • [7] BOYAN BD, 1992, CLIN ORTHOP RELAT R, V278, P286
  • [8] BRIGHTON CT, 1992, CLIN ORTHOP RELAT R, V275, P287
  • [9] OSTEOGENIC CELL LINEAGE ANALYSIS IS FACILITATED BY ORGAN-CULTURES OF EMBRYONIC CHICK PERIOSTEUM
    BRUDER, SP
    CAPLAN, AI
    [J]. DEVELOPMENTAL BIOLOGY, 1990, 141 (02) : 319 - 329
  • [10] Bruder SP, 1998, CLIN ORTHOP RELAT R, pS247