Induction of MafBx and Murf ubiquitin ligase mRNAs in rat skeletal muscle after LPS injection

被引：122

作者：

Dehoux, MJM ^{[1
]}

van Beneden, RP ^{[1
]}

Fernández-Celemín, L ^{[1
]}

Lause, PL ^{[1
]}

Thissen, JPM ^{[1
]}

机构：

[1] Univ Catholique Louvain, Unite Diabetol & Nutr, B-1200 Brussels, Belgium

来源：

FEBS LETTERS | 2003年 / 544卷 / 1-3期

关键词：

muscle; ubiquitin ligase; tumor necrosis factor-alpha; insulin like growth factor-1; lipopolysaccharide; real-time quantitative polymerase chain reaction;

D O I：

10.1016/S0014-5793(03)00505-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MafBx and Murf are two new rat E3 ubiquitin ligases induced in muscle atrophy. Our goal was to investigate whether lipopolysaccharide (LPS) injection, a model of muscle catabolism, is associated with increased expression of MafBx and Murf. LPS (750 mug/100 g body weight) induces MafBx and Murf mRNA (respectively, 23-fold and 33-fold after 12 h; P < 0.001). A transient induction of tumor necrosis factor-alpha mRNA (21-fold; P < 0.001 at 3 h) and a decrease of insulin like growth factor-I mRNA (50%; P < 0.001 at 6 h), two potential regulators of the ubiquitin-proteasome system were also demonstrated. In summary, MafBx and Murf mRNA are upregulated in response to LPS and might play a role in the muscle proteolysis observed. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：214 / 217

页数：4

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