Multilocus sequence typing of Staphylococcus aureus with DNA array technology

被引:65
作者
van Leeuwen, WB
Jay, C
Snijders, S
Durin, N
Lacroix, B
Verbrugh, HA
Enright, MC
Troesch, A
van Belkum, A
机构
[1] Erasmus MC, Dept Med Microbiol & Infect Dis, NL-3015 GD Rotterdam, Netherlands
[2] BioMerieux, Marcy Letoile, France
[3] Univ Bath, Bath BA2 7AY, Avon, England
关键词
D O I
10.1128/JCM.41.7.3323-3326.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant.
引用
收藏
页码:3323 / 3326
页数:4
相关论文
共 20 条
  • [1] Genome and virulence determinants of high virulence community-acquired MRSA
    Baba, T
    Takeuchi, F
    Kuroda, M
    Yuzawa, H
    Aoki, K
    Oguchi, A
    Nagai, Y
    Iwama, N
    Asano, K
    Naimi, T
    Kuroda, H
    Cui, L
    Yamamoto, K
    Hiramatsu, K
    [J]. LANCET, 2002, 359 (9320) : 1819 - 1827
  • [2] Usefulness of multilocus sequence typing for characterization of clinical isolates of Candida albicans
    Bougnoux, ME
    Morand, S
    d'Enfert, C
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (04) : 1290 - 1297
  • [3] Database-driven multi locus sequence typing (MLST) of bacterial pathogens
    Chan, MS
    Maiden, MCJ
    Spratt, BG
    [J]. BIOINFORMATICS, 2001, 17 (11) : 1077 - 1083
  • [4] The evolution of methicillin resistance in Staphylococcus aureus:: Similarity of genetic backgrounds in historically early methicillin-susceptible and -resistant isolates and contemporary epidemic clones
    Crisóstomo, MI
    Westh, H
    Tomasz, A
    Chung, M
    Oliveira, DC
    de Lencastre, H
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) : 9865 - 9870
  • [5] Using DNA microarrays to study host-microbe interactions
    Cummings, CA
    Relman, DA
    [J]. EMERGING INFECTIOUS DISEASES, 2000, 6 (05) : 513 - 525
  • [6] Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci
    Dunman, PM
    Murphy, E
    Haney, S
    Palacios, D
    Tucker-Kellogg, G
    Wu, S
    Brown, EL
    Zagursky, RJ
    Shlaes, D
    Projan, SJ
    [J]. JOURNAL OF BACTERIOLOGY, 2001, 183 (24) : 7341 - 7353
  • [7] Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus
    Enright, MC
    Day, NPJ
    Davies, CE
    Peacock, SJ
    Spratt, BG
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (03) : 1008 - 1015
  • [8] MULTIPLEXED BIOCHEMICAL ASSAYS WITH BIOLOGICAL CHIPS
    FODOR, SPA
    RAVA, RP
    HUANG, XHC
    PEASE, AC
    HOLMES, CP
    ADAMS, CL
    [J]. NATURE, 1993, 364 (6437) : 555 - 556
  • [9] Gingeras TR, 2000, ASM NEWS, V66, P463
  • [10] MOLECULAR TYPING OF STAPHYLOCOCCUS-AUREUS ON THE BASIS OF COAGULASE GENE POLYMORPHISMS
    GOH, SH
    BYRNE, SK
    ZHANG, JL
    CHOW, AW
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) : 1642 - 1645